Interleukin 1β and tumour necrosis factor α secreting cells are increased in the peripheral blood of patients with primary Sjögren’s syndrome

Objective: To study systemic alterations of cytokine secreting peripheral blood mononuclear cells (PBMC) in primary Sjögren’s syndrome (pSS) and their relation to common clinical and immunological manifestations of this disease. Methods: PBMC spontaneously secreting tumour necrosis factor α (TNFα),...

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Bibliographic Details
Published in:Annals of the rheumatic diseases 2003-04, Vol.62 (4), p.359-362
Main Authors: Willeke, P, Schotte, H, Schlüter, B, Erren, M, Becker, H, Dyong, A, Mickholz, E, Domschke, W, Gaubitz, M
Format: Article
Language:English
Subjects:
Biological and medical sciences
bovine serum albumin
BSA
Cytokines
ELISA
ELISPOT
enzyme linked immunosorbent assay
enzyme linked immunospot
erythrocyte sedimentation rate
ESR
Immune response
interleukin
interleukin 1β
Interleukin-1
labial salivary gland
LSG
Medical sciences
PBMC
PBS
peripheral blood mononuclear cells
Peripheral circulation
phosphate buffered saline
Physiological aspects
primary Sjögren’s syndrome
pSS
recurrent parotid swelling
rheumatoid factor
RPS
Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis
Sjogren's syndrome
Sjögren’s syndrome
TNFα
Tumor necrosis factor
tumour necrosis factor α
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Summary:Objective: To study systemic alterations of cytokine secreting peripheral blood mononuclear cells (PBMC) in primary Sjögren’s syndrome (pSS) and their relation to common clinical and immunological manifestations of this disease. Methods: PBMC spontaneously secreting tumour necrosis factor α (TNFα), interleukin 1β (IL1β), and interleukin 6 (IL6) were assessed by enzyme linked immunospot (ELISPOT) analysis in a cohort of 31 patients with pSS fulfilling the modified European classification criteria. Nineteen healthy volunteers served as controls. ELISPOT results were correlated with glandular and extraglandular manifestations and autoantibody titres—that is, rheumatoid factor (RF) isotypes, anti-Ro/SS-A, anti-La/SS-B as determined by an enzyme linked immunosorbent assay (ELISA) technique. Results: The number of TNFα and IL1β secreting cells was significantly higher in patients with pSS than in controls. No differences were detected in the number of IL6 secreting PBMC. Patients with recurrent parotid swelling (RPS) had a significantly increased number of IL1β secreting PBMC. Moreover, the number of IL1β secreting PBMC correlated with the disease duration (rs=0.479; p
ISSN:0003-4967
1468-2060
DOI:10.1136/ard.62.4.359