Phagocytes as Carcinogens: Malignant Transformation Produced by Human Neutrophils

In a study of the relation between chronic inflammation and carcinogenesis, C3H mouse fibroblasts of the 10T 1/2 clone 8 line (10T 1/2 cells) were exposed to human neutrophils stimulated to synthesize reactive oxygen intermediates or to a cell-free enzymatic system generating superoxide (xanthine ox...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1985-03, Vol.227 (4691), p.1231-1233
Main Authors: Weitzman, Sigmund A., Weitberg, Alan B., Clark, Edward P., Stossel, Thomas P.
Format: Article
Language:English
Subjects:
3T3 cells
Animals
Biological and medical sciences
Cancer
Cancer research
Carcinogenesis, carcinogens and anticarcinogens
Carcinogens
Cell lines
Cell Transformation, Neoplastic - immunology
Cocarcinogens
General aspects
Humans
Lesions
Medical sciences
Mice
Mice, Inbred C3H
Mice, Nude
Neutrophils
Neutrophils - metabolism
Neutrophils - physiology
Oncology, Experimental
Oxidases
Oxidation-Reduction - drug effects
Phagocytes
Phagocytes - physiology
Tetradecanoylphorbol Acetate - pharmacology
Transformed cell line
Tumor cell line
Tumors
Xanthines
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In a study of the relation between chronic inflammation and carcinogenesis, C3H mouse fibroblasts of the 10T 1/2 clone 8 line (10T 1/2 cells) were exposed to human neutrophils stimulated to synthesize reactive oxygen intermediates or to a cell-free enzymatic system generating superoxide (xanthine oxidase plus hypoxanthine). After exposure, the 10T 1/2 cells were either placed in tissue culture or immediately injected into athymic nude mice. Both malignant and benign tumors developed in the mice injected with treated cells, but not in those injected with control cells; in one instance cells grown from one of the benign tumors subsequently developed a malignant phenotype. Malignant transformation was also observed in treated cells in the experiments in vitro.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.3975611