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A [Β.sub.2] Adrenergic Receptor Signaling Complex Assembled with the [Ca.sup.2+] Channel [Ca.sub.v] 1.2
The existence of a large number of receptors coupled to heterotrimeric guanine nucleotide binding proteins (G proteins) raises the question of how a particular receptor selectively regulates specific targets. We provide insight into this question by identifying a prototypical macromolecular signalin...
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Published in: | Science (American Association for the Advancement of Science) 2001-07, Vol.293 (5527), p.98 |
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Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
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Summary: | The existence of a large number of receptors coupled to heterotrimeric guanine nucleotide binding proteins (G proteins) raises the question of how a particular receptor selectively regulates specific targets. We provide insight into this question by identifying a prototypical macromolecular signaling complex. The [Β.sub.2] adrenergic receptor was found to be directly associated with one of its ultimate effectors, the class C L-type calcium channel C[a.sub.v]1.2. This complex also contained a G protein, an adenylyl cyclase, cyclic adenosine monophosphate-dependent protein kinase, and the counterbalancing phosphatase PP2A. Our electrophysiological recordings from hippocampal neurons demonstrate highly localized signal transduction from the receptor to the channel. The assembly of this signaling complex provides a mechanism that ensures specific and rapid signaling by a G protein-coupled receptor. |
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ISSN: | 0036-8075 1095-9203 |