Amphiphysin 2

In striated muscle, the plasma membrane forms tubular invaginations (transverse tubules or T-tubules) that function in depolarization-contraction coupling. Caveolin-3 and amphiphysin were implicated in their biogenesis. Amphiphysin isoforms have a putative role in membrane deformation at endocytic s...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2002-08, Vol.297 (5584), p.1193
Main Authors: Lee, Eunkyung, Marcucci, Melissa, Daniell, Laurie, Pypaert, Marc, Weisz, Ora A, Ochoa, Gian-Carlo, Farsad, Khashayar, Wenk, Markus R, De Camilli, Pietro
Format: Article
Language:English
Subjects:
Cell membranes
Developmental cytology
Muscles
Physiological aspects
Online Access:Get full text
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Summary:In striated muscle, the plasma membrane forms tubular invaginations (transverse tubules or T-tubules) that function in depolarization-contraction coupling. Caveolin-3 and amphiphysin were implicated in their biogenesis. Amphiphysin isoforms have a putative role in membrane deformation at endocytic sites. An isoform of amphiphysin 2 concentrated at T-tubules induced tubular plasma membrane invaginations when expressed in nonmuscle cells. This property required exon 10, a phosphoinositide-binding module. In developing myotubes, amphiphysin 2 and caveolin-3 segregated in tubular and vesicular portions of the T-tubule system, respectively. These findings support a role of the bilayer-deforming properties of amphiphysin at T-tubules and, more generally, a physiological role of amphiphysin in membrane deformation.
ISSN:0036-8075
1095-9203