Histomorphological changes in murine fibrosarcoma after hypericin-based photodynamic therapy

Histomorphological changes in murine fibrosarcoma after photodynamic therapy (PDT) based on the natural photosensitizer hypericin were evaluated. C3H/DiSn mice were inoculated with fibrosarcoma G5:1:13 cells. When the tumour reached a volume of 40–80 mm 3 the mice were intraperitoneally injected wit...

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Published in:Phytomedicine (Stuttgart) 2007-02, Vol.14 (2), p.172-178
Main Authors: Bobrov, Nikita, Čavarga, Ivan, Longauer, František, Rybárová, Silvia, Fedoročko, Peter, Brezáni, Peter, Miškovský, Pavol, Mirossay, Ladislav, Štubňa, Ján
Format: Article
Language:English
Subjects:
Animals
Antineoplastic Agents, Phytogenic - administration & dosage
Antineoplastic Agents, Phytogenic - pharmacology
Antineoplastic Agents, Phytogenic - therapeutic use
Cancer
Cancerotherapy
Care and treatment
Cell Line, Tumor - drug effects
Disease Models, Animal
Fibrosarcoma
Fibrosarcoma - drug therapy
Fibrosarcoma - pathology
Humans
Hypericin
Hypericum
Injections, Intraperitoneal
Male
Mice
Mice, Inbred C3H
Patient outcomes
Perylene - administration & dosage
Perylene - analogs & derivatives
Perylene - pharmacology
Perylene - therapeutic use
Photochemotherapy
Photodynamic therapy
Photosensitizing Agents - administration & dosage
Photosensitizing Agents - pharmacology
Photosensitizing Agents - therapeutic use
Phytotherapy
Plant Extracts - administration & dosage
Plant Extracts - pharmacology
Plant Extracts - therapeutic use
Radiation Dosage
Tumour
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Summary:Histomorphological changes in murine fibrosarcoma after photodynamic therapy (PDT) based on the natural photosensitizer hypericin were evaluated. C3H/DiSn mice were inoculated with fibrosarcoma G5:1:13 cells. When the tumour reached a volume of 40–80 mm 3 the mice were intraperitoneally injected with hypericin, either in a single dose (5 mg/kg; 1 or 6 h before laser irradiation) or two fractionated doses (2.5 mg/kg; 6 and 1 h before irradiation with laser light; 532 nm, 70 mW/cm 2, 168 J/cm 2). All groups of PDT-treated animals with single and fractionated hypericin dosing presented primary vascular reactions including vascular dilatation, congestion, thrombosis and oedema. Two hours after PDT there were necrotic changes with small, rather focal appearance. One day after therapy the necrotic areas were enhanced, often affecting a complete superficial layer of tumour tissue. Necrotic areas were accompanied with inflammation and haemorrhages.
ISSN:0944-7113
1618-095X
DOI:10.1016/j.phymed.2006.09.017