Isolation and characterisation of micro-satellites from hexaploid bread wheat

The development of large panels of simple-to-analyse genetic markers for tagging agronomically important genes and diversity studies in hexaploid bread wheat is an important goal in applied cereal genetic research. We have isolated and sequenced over 200 clones containing micro-satellites from the w...

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Bibliographic Details
Published in:Theoretical and applied genetics 1997-04, Vol.94 (5), p.557
Main Authors: Bryan, G.J, Collins, A.J, Stephenson, P, Orry, A, Smith, J.B, Gale, M.D
Format: Article
Language:English
Subjects:
Genetic aspects
Genetic polymorphisms
Identification and classification
Microsatellites (Genetics)
Plant genetics
Wheat
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Summary:The development of large panels of simple-to-analyse genetic markers for tagging agronomically important genes and diversity studies in hexaploid bread wheat is an important goal in applied cereal genetic research. We have isolated and sequenced over 200 clones containing micro-satellites from the wheat genome and have tested 153 primer pairs for genetic polymorphism using a panel of ten wheat varieties, including the parents of our main mapping cross. A subset comprising 49 primer pairs detects 76 loci, of which 74 can be unequivocably allocated to one of the wheat chromosomes. A relatively low frequency of the loci detected are from the D genome, and these loci show less polymorphism than those from the A and B genomes. Generally, the micro-satellites show high levels of genetic polymorphism and an average of 3.5 alleles per locus with an average polymorphism information content (PIC), value of 0.51. The observed levels of polymorphism are positively correlated with the length of the micro-satellite repeats. A high proportion, approximately two-thirds, of primer pairs designed to detect simple sequence repeat (SSR) variation in wheat do not generate the expected amplification products and, more significantly, often generate unresolvable PCR products. In general, our results agree closely with those obtained from other recent studies using micro-satellites in plants.
ISSN:0040-5752