Molecular identification of Cordylobia anthropophaga Blanchard in Jos South, Plateau State, Nigeria

Myiasis-causing larvae were extracted from dogs attending veterinary clinics in Plateau State, Nigeria and subjected to molecular analysis involving polymerase chain reaction amplification of the 28S rRNA gene of blowflies, cloning and sequencing techniques. All larvae were confirmed as Cordylobia a...

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Bibliographic Details
Published in:Onderstepoort journal of veterinary research 2012-01, Vol.79 (1)
Main Authors: Ogo, Ndudim I, Onovoh, Emmanuel, Okubanjo, Oluyinka O, Galindo, Ruth C, de la Lastra, Jose-Manuel P, de la Fuente, Jose
Format: Article
Language:English
Subjects:
Blowflies
Dogs
RNA
Skin
Online Access:Get full text
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Summary:Myiasis-causing larvae were extracted from dogs attending veterinary clinics in Plateau State, Nigeria and subjected to molecular analysis involving polymerase chain reaction amplification of the 28S rRNA gene of blowflies, cloning and sequencing techniques. All larvae were confirmed as Cordylobia anthropophaga Blanchard (Diptera: Calliphoridae) after the initial morphological identification. This is the first molecular identification of any myiasis-causing fly species in Nigeria and may serve as a reliable alternative to morphological identification where samples are not well preserved or difficult to identify to species level.
ISSN:0030-2465
DOI:10.4102/ojvr.v79i1.349