Effect of N-acetyl cysteine (NAC), an organosulfur compound from Allium plants, on experimentally induced hepatic prefibrogenic events in wistar rat

Aim of present study was to investigate the effect of NAC on experimental chronic hepatotoxicity models induced by carbon tetrachloride (CCl4) and thioacetamide (TAA). CCl4 toxicity was induced by administering 200μl CCl4 (diluted 2:3 in coconut oil)/100g body weight, p.o., twice weekly for 8 weeks....

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Bibliographic Details
Published in:Phytomedicine (Stuttgart) 2013-07, Vol.20 (10), p.828-833
Main Authors: Nissar, Ashraf U., Farrukh, Mufti R., Kaiser, Peerzada J., Rafiq, Rather A., Afnan, Quadri, Bhushan, Shashi, Adil, Hassan S., Subhash, Bhardjwaj C., Tasduq, Sheikh A.
Format: Article
Language:English
Subjects:
Acetylcysteine - pharmacology
Acetylcysteine - therapeutic use
Allium
Animals
Carbon tetrachloride
Carbon Tetrachloride - administration & dosage
Cysteine
Disease Models, Animal
Fibrosis
Health aspects
Liver
Liver Cirrhosis - chemically induced
Liver Cirrhosis - pathology
Liver Cirrhosis - prevention & control
Male
N-acetyl cysteine from Allium plants
Oxidative Stress - drug effects
Physiological aspects
Phytochemistry
Phytotherapy
Rats
Rats as laboratory animals
Rats, Wistar
Sulfur - administration & dosage
Thioacetamide
Thioacetamide - administration & dosage
Wistar rat
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Summary:Aim of present study was to investigate the effect of NAC on experimental chronic hepatotoxicity models induced by carbon tetrachloride (CCl4) and thioacetamide (TAA). CCl4 toxicity was induced by administering 200μl CCl4 (diluted 2:3 in coconut oil)/100g body weight, p.o., twice weekly for 8 weeks. TAA toxicity was induced by administering 150mg/kg b. wt. of TAA i.p., twice weekly for 8 weeks. NAC treatment was started along with toxicants (CCl4 and TAA) for 8 weeks and continued for further 4 weeks. Self reversal group was kept without any treatment for 4 weeks after completion of toxicant treatments. Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP), Bilirubin were measured in serum. Hydroxyproline (HP), lipid peroxidation (LPO), catalase (CAT), Glutathione peroxidase (GPx) and Glutathione (GSH) were determined in liver samples by colorimetric methods. Cytochrome P450 2E1 (CYP 450 2E1), activity was determined as hydroxylation of aniline in liver microsomes. General examination and histological analysis were also performed. Serum markers of liver damage (AST, ALT, ALP and Bilirubin) were increased by CCl4 and TAA intoxication (p
ISSN:0944-7113
1618-095X
DOI:10.1016/j.phymed.2013.03.009