Phosphate flow between hybrid histidine kinases Che[A.sub.3] and Che[S.sub.3] controls rhodospirillum centenum cyst formation

Genomic and genetic analyses have demonstrated that many species contain multiple chemotaxis-like signal transduction cascades that likely control processes other than chemotaxis. The [Che.sub.3] signal transduction cascade from Rhodospirillum centenum is one such example that regulates development...

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Bibliographic Details
Published in:PLoS genetics 2013-12, Vol.9 (12)
Main Authors: He, Kuang, Marden, Jeremiah N, Quardokus, Ellen M, Bauer, Carl E
Format: Article
Language:English
Subjects:
Cellular signal transduction
Genetic aspects
Genetic research
Histidine
Phosphotransferases
Physiological aspects
Rhizosphere
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Summary:Genomic and genetic analyses have demonstrated that many species contain multiple chemotaxis-like signal transduction cascades that likely control processes other than chemotaxis. The [Che.sub.3] signal transduction cascade from Rhodospirillum centenum is one such example that regulates development of dormant cysts. This Che-like cascade contains two hybrid response regulator-histidine kinases, Che[A.sub.3] and Che[S.sub.3], and a single-domain response regulator Che[Y.sub.3]. We demonstrate that Che[S.sub.3] is epistatic to Che[A.sub.3] and that only Che[S.sub.3]~P can phosphorylate Che[Y.sub.3]. We further show that Che[A.sub.3] derepresses cyst formation by phosphorylating a Che[S.sub.3] receiver domain. These results demonstrate that the flow of phosphate as defined by the paradigm E. coli chemotaxis cascade does not necessarily hold true for non-chemotactic Che-like signal transduction cascades.
ISSN:1553-7390
1553-7404
DOI:10.1371/journal.pgen.1004002