Fine mapping of [qSTV11.sup.KAS], a major QTL for rice stripe disease resistance

Rice stripe disease, caused by rice stripe virus (RSV), is one of the most serious diseases in temperate rice-growing areas. In the present study, we performed quantitative trait locus (QTL) analysis for RSV resistance using 98 backcross inbred lines derived from the cross between the highly resista...

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Bibliographic Details
Published in:Theoretical and applied genetics 2011-05, Vol.122 (8), p.1591
Main Authors: Zhang, Ying-Xin, Wang, Qi, Jiang, Ling, Liu, Ling-Long, Wang, Bao-Xiang, Shen, Ying-Yue, Cheng, Xia-Nian, Wan, Jian- min
Format: Article
Language:English
Subjects:
Analysis
Diseases and pests
Genetic aspects
Physiological aspects
Plant viruses
Quantitative trait loci
Rice
Virus diseases of plants
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Summary:Rice stripe disease, caused by rice stripe virus (RSV), is one of the most serious diseases in temperate rice-growing areas. In the present study, we performed quantitative trait locus (QTL) analysis for RSV resistance using 98 backcross inbred lines derived from the cross between the highly resistant variety, Kasalath, and the highly susceptible variety, Nipponbare. Under artificial inoculation in the greenhouse, two QTLs for RSV resistance, designated qSTV7 and [qSTV11.sup.KAS,] were detected on chromosomes 7 and 11 respectively, whereas only one QTL was detected in the same location of chromosome 11 under natural inoculation in the field. The stability of [qSTV11.sup.KAS] was validated using 39 established chromosome segment substitution lines. Fine mapping of [qSTV11.sup.KAS] was carried out using 372 B[C.sub.3][F.sub.2:3] recombinants and 399 B[C.sub.3][F.sub.3:4] lines selected from 7,018 B[C.sub.3][F.sub.2] plants of the cross SL-234/Koshihikari. The [qSTV11.sup.KAS] was localized to a 39.2 kb region containing seven annotated genes. The most likely candidate gene, LOC_Os11g30910, is predicted to encode a sulfotransferase domain-containing protein. The predicted protein encoded by the Kasalath allele differs from Nipponbare by a single amino acid substitution and the deletion of two amino acids within the sulfotransferase domain. Marker-resistance association analysis revealed that the markers L104-155 bp and R48-194 bp were highly correlated with RSV resistance in the 148 landrace varieties. These results provide a basis for the cloning of [qSTV11.sup.KAS], and the markers may be used for molecular breeding of RSV resistant rice varieties.
ISSN:0040-5752
1432-2242
DOI:10.1007/s00122-011-1557-0