Determination of dihydroxymelphalan in perfusate, blood plasma, and lung tissue by HPLC-MS: Use in a pharmacokinetic study

s A rapid, sensitive, and selective method for determining dihydroxymelphalan ( DHM ) in biological samples by HPLC-MS is developed. Sample pretreatment of blood plasma, perfusate, and lung tissue includes the steps of hydrolysis, homogenization (for tissue), and protein precipitation. The recovery...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2014-04, Vol.69 (4), p.377-383
Main Authors: Sidorova, A. A., Grigoriev, A. V., Timofeeva, E. S., Levchenko, E. V.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Antimitotic agents
Antineoplastic agents
Chemical properties
Chemistry
Chemistry and Materials Science
High performance liquid chromatography
Mass spectrometry
Pharmacokinetics
Physiological aspects
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Summary:s A rapid, sensitive, and selective method for determining dihydroxymelphalan ( DHM ) in biological samples by HPLC-MS is developed. Sample pretreatment of blood plasma, perfusate, and lung tissue includes the steps of hydrolysis, homogenization (for tissue), and protein precipitation. The recovery of DHM, is 91.2 ± 4.0, 89.5 ± 2.7, and 75.9 ± 3.8% for perfusion solution, blood plasma, and lung tissue, respectively. The detection limit for DHM is 1.0 ng/mL in perfusate and blood plasma and 2.0 ng/mL in tissue. The method was applied to the study of the pharmacokinetics of an antitumor drug Alkeran after the intraoperative isolated chemoperfusion of lung.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934814040108