Determination of dihydroxymelphalan in perfusate, blood plasma, and lung tissue by HPLC-MS: Use in a pharmacokinetic study

s A rapid, sensitive, and selective method for determining dihydroxymelphalan ( DHM ) in biological samples by HPLC-MS is developed. Sample pretreatment of blood plasma, perfusate, and lung tissue includes the steps of hydrolysis, homogenization (for tissue), and protein precipitation. The recovery...

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Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2014-04, Vol.69 (4), p.377-383
Main Authors: Sidorova, A. A., Grigoriev, A. V., Timofeeva, E. S., Levchenko, E. V.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Antimitotic agents
Antineoplastic agents
Chemical properties
Chemistry
Chemistry and Materials Science
High performance liquid chromatography
Mass spectrometry
Pharmacokinetics
Physiological aspects
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container_issue 4
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container_title Journal of analytical chemistry (New York, N.Y.)
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creator Sidorova, A. A.
Grigoriev, A. V.
Timofeeva, E. S.
Levchenko, E. V.
description s A rapid, sensitive, and selective method for determining dihydroxymelphalan ( DHM ) in biological samples by HPLC-MS is developed. Sample pretreatment of blood plasma, perfusate, and lung tissue includes the steps of hydrolysis, homogenization (for tissue), and protein precipitation. The recovery of DHM, is 91.2 ± 4.0, 89.5 ± 2.7, and 75.9 ± 3.8% for perfusion solution, blood plasma, and lung tissue, respectively. The detection limit for DHM is 1.0 ng/mL in perfusate and blood plasma and 2.0 ng/mL in tissue. The method was applied to the study of the pharmacokinetics of an antitumor drug Alkeran after the intraoperative isolated chemoperfusion of lung.
doi_str_mv 10.1134/S1061934814040108
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subjects Analytical Chemistry
Antimitotic agents
Antineoplastic agents
Chemical properties
Chemistry
Chemistry and Materials Science
High performance liquid chromatography
Mass spectrometry
Pharmacokinetics
Physiological aspects
title Determination of dihydroxymelphalan in perfusate, blood plasma, and lung tissue by HPLC-MS: Use in a pharmacokinetic study
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