Spectroscopic study of interactions between dye molecules in micelle and liposome nanovolumes

The efficiency of Förster resonance energy transfer (FRET) between pairs of different dyes in the nanovolumes of surfactant micelles and liposomes was studied using optical spectroscopy methods. More efficient FRET with the same solution concentrations of the dyes was observed in liposomes despite t...

Full description

Saved in:
Bibliographic Details
Published in:Journal of applied spectroscopy 2013, Vol.79 (6), p.914-921
Main Authors: Yefimova, S. L., Tkacheva, T. N., Kurilchenko, I. Yu, Sorokin, A. V., Malyukin, Yu. V.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Atomic/Molecular Structure and Spectra
Comparative analysis
Membrane lipids
Physics
Physics and Astronomy
Spectrum analysis
Surface active agents
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The efficiency of Förster resonance energy transfer (FRET) between pairs of different dyes in the nanovolumes of surfactant micelles and liposomes was studied using optical spectroscopy methods. More efficient FRET with the same solution concentrations of the dyes was observed in liposomes despite the greater diameter of liposomes compared with micelles (100 and 5 nm). Calculations showed that the observed effect was explained by the smaller amount of liposomes in the solution and, consequently, the larger local concentration of the dyes in the liposome lipid bilayer. It was shown that not only the spectral overlap integral and the Förster distance but also specific interactions between molecules in the nanovolume (electrostatic, vander-Waals, and steric) that were governed by structural features of the molecules affected the FRET efficiency.
ISSN:0021-9037
1573-8647
DOI:10.1007/s10812-013-9694-7