Determination of acysole in whole blood by high-performance liquid chromatography

A method is developed for the determination of a carbon monoxide antidote acysole in whole blood using hydrophilic interaction high-performance liquid chromatography with UV detection. Two methods were used for sample preparation: protein precipitation with acetonitrile and solid-phase extraction (S...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2014-08, Vol.69 (8), p.800-804
Main Authors: Kursakov, S. V., Belov, V. Yu, Sevast’yanov, V. I.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Antidotes
Blood
Chemical properties
Chemistry
Chemistry and Materials Science
Composition
High performance liquid chromatography
Identification and classification
Methods
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Summary:A method is developed for the determination of a carbon monoxide antidote acysole in whole blood using hydrophilic interaction high-performance liquid chromatography with UV detection. Two methods were used for sample preparation: protein precipitation with acetonitrile and solid-phase extraction (SPE) with an acysole recovery of 95.8 and 89.8%, respectively. Chromatographic determination was performed in the isocratic mode on a Nucleodur HILIC column and with an acetonitrile: water 95: 5 mobile phase at 225 nm. The calibration graphs are linear in the concentration range 0.056–111.1 μg/mL. The limits of detection and determination of acysole were 0.050 and 0.120 μg/mL after protein precipitation with acetonitrile and 0.071 and 0.169 μg/mL with SPE, respectively.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934814060094