In vivo biodistribution of pancreatic-derived factor using [sup.18]F-labeled PANDER PET imaging

• Published in: Journal of radioanalytical and nuclear chemistry 2014-08, Vol.301 (2), p.333
• Main Authors: Lai, Feng Hua, Tang, Gang Hua, Yang, Chi Jiao, Wang, Hong Liang, Hu, Kong Zhen, Cao, Xiao Pei
• Format: Article
• Language: English
• Subjects: Organic compounds; PET imaging; Sarcoma; Synthesis
• ISSN: 0236-5731
• DOI: 10.1007/s10967-014-3181-3

The purpose of this study was to investigate in vivo biodistribution and potential target tissues of pancreatic-derived factor (PANDER, FAM3B) using [sup.18]Flabeled PANDER positron emission tomography (PET) imaging. [sup.18]F-Labeled PANDER ([[sup.18]F]FB-PANDER) was prepared by reaction of PANDER and N-succinimidyl-4[[sup.18]F]fluorobenzoate ([[sup.18]F]SFB). The uncorrected radiochemical yield of [[sup.18]F]FB-PANDER was 15.2 ± 3.4 % (n = 4) based on [[sup.18]F]SFB within the total synthesis time of 30 min. In vivo biodistribution of [[sup.18]F]FB-PANDER in nomal mice and PET imaging demonstrated high uptake of the radiotracer in urinary bladder, kidneys and gall bladder, and fast clearance from kidneys and gall bladder. Also, moderate uptake in blood, liver, pancreas, small intestine and bone, low uptake in brain and muscle, and almost no uptake in S180 fibrosarcoma tissue were observed. The results indicated that the major excretion route of PANDER was through renal-urinary bladder and biliary system, and no obvious binding targets of PANDER in the main organs and S180 fibrosarcoma tissue were found. Keywords PANDER * [[sup.18]F]SFB * Radiosynthesis * Biodistribution * Target tissue * PET imaging