Synthesis, characterization and biodistribution of [sup.99m]Tc-bioquin-HMPAO as a novel brain imaging agent

• Published in: Journal of radioanalytical and nuclear chemistry 2014-10, Vol.302 (1), p.563
• Main Authors: Kilcar, Ayfer Yurt, Muftuler, Fazilet Zumrut Biber, Enginar, Huseyin, Tekin, Volkan, Medine, Emin Ilker, Unak, Perihan
• Format: Article
• Language: English
• Subjects: Brain; Chemical properties; High performance liquid chromatography; Quality control
• ISSN: 0236-5731
• DOI: 10.1007/s10967-014-3175-1

Recently, the development of novel brain imaging agents has aroused much interest thanks to limited number of brain cancer or diseases diagnosis agents. It is aimed to synthesize a novel brain imaging agent including a promise for further studies on AD diagnosis potential and investigate its bioaffinity with biodistribution studies on healthy Balb/c mice. A novel radiolabeled agent was synthesized and characterized. Quality control of [sup.99m]Tc-BH was performed utilizing solvent extraction and chromatographic (Radio TLC and Radio HPLC) methods. Bioaffinity of the [sup.99m]Tc-BH was investigated on male Balb/c mice at various time points (5, 30, 60, 120 min post-injection). Paper electrophoresis showed that [sup.99m]Tc-BH has a neutral structure. Radiochemical purity of [sup.99m]Tc-BH was over 95 % with appropriate stability for imaging period. Selected brain regions have uptakes over 4 % ID/g following intravenous injection. Hippocampus has uptake approximately 10 % ID/g. [sup.99m]Tc-BH has shown brain uptake, so it may prove to be valuable for brain imaging as a novel technetium-labeled agent. Further investigations with AD animal model are our on going effort to show that this agent has AD diagnosis potential. Keywords Brain imaging * HMPAO * Bioquin * [sup.99m]Tc