3-Deoxygalactosone, a new glucose degradation product in peritoneal dialysis fluids: Identification, quantification by HPLC/DAD/MSMS and its pathway of formation Stefan Mittelmaier Michael Funfrocken Dominik Fenn Monika Pischetsrieder

Heat sterilization of peritoneal dialysis (PD) fluids leads to the formation of glucose degradation products (GDPs), which considerably impair long-term application of PD. Knowledge of the exact composition of GDPs present in a PD fluid is important to improve the biocompatibility of dialysis soluti...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2011-02, Vol.399 (4), p.1689
Main Authors: Mittelmaier, Stefan, Funfrocken, Michael, Fenn, Dominik, Pischetsrieder, Monika
Format: Article
Language:English
Subjects:
Analysis
By-products
Carbonyl compounds
Chemical properties
Complications and side effects
Contamination
Continuous ambulatory peritoneal dialysis
Decomposition (Chemistry)
Dextrose
Equipment and supplies
Glucose
High performance liquid chromatography
Hypertonic solutions
Identification and classification
Mass spectrometry
Methods
Peritoneal dialysis
Physiological aspects
Thermal properties
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Summary:Heat sterilization of peritoneal dialysis (PD) fluids leads to the formation of glucose degradation products (GDPs), which considerably impair long-term application of PD. Knowledge of the exact composition of GDPs present in a PD fluid is important to improve the biocompatibility of dialysis solutions. The present study conducted a targeted screening for novel GDPs with α-dicarbonyl structure in PD fluids. Thus, 3-deoxygalactosone (3-DGal) was identified for the first time in PD fluids. Quantification of 3-DGal was achieved by high-performance liquid chromatography (HPLC)/DAD/ MSMS after derivatization with o-phenylendiamine to yield the quinoxaline derivative. Baseline separation of all α-dicarbonyl GDPs, particularly of the diastereomers 3-deoxyglucosone (3-DG) and 3-DGal, required the application of a polar, phenyl-based RP column for HPLC and additional pH-gradient elution. Concentrations of 3-DGal ranged between 55.8 and 136.9 μM in single-chamber PD fluids, and between 2.5 and 12.4 μM in double-chamber PD fluids. In solutions containing glucose, 3-DGal is formed from 3-DG via the intermediate 3,4-dideoxyglucosone-3-ene (3,4-DGE). Further studies are now required to determine the (patho-)physiological properties of 3-DGal. Keywords α-Dicarbonyl compounds * Glucose degradation products (GDPs) * 3-Deoxygalactosone (3-DGal) * HPLC * Peritoneal dialysis fluid * Quinoxaline derivatives
ISSN:1618-2642
DOI:10.1007/s00216-010-4456-3