Mechanisms of Intestinal Serotonin Transporter (SERT) Upregulation by TGF-[beta]1 Induced Non-Smad Pathways

TGF-[beta]1 is an important multifunctional cytokine with numerous protective effects on intestinal mucosa. The influence of TGF-[beta]1 on serotonin transporter (SERT) activity, the critical mechanism regulating the extracellular availability of serotonin (5-HT), is not known. Current studies were...

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Bibliographic Details
Published in:PloS one 2015-05, Vol.10 (5)
Main Authors: Nazir, Saad, Kumar, Anoop, Chatterjee, Ishita, Anbazhagan, Arivarasu N, Gujral, Tarunmeet, Priyamvada, Shubha, Saksena, Seema, Alrefai, Waddah A, Dudeja, Pradeep K, Gill, Ravinder K
Format: Article
Language:English
Subjects:
Bone morphogenetic proteins
Monomolecular films
Phenols (Class of compounds)
Serotonin
Transforming growth factors
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Summary:TGF-[beta]1 is an important multifunctional cytokine with numerous protective effects on intestinal mucosa. The influence of TGF-[beta]1 on serotonin transporter (SERT) activity, the critical mechanism regulating the extracellular availability of serotonin (5-HT), is not known. Current studies were designed to examine acute effects of TGF-[beta]1 on SERT. Model human intestinal Caco-2 cells grown as monolayer's or as cysts in 3D culture and ex vivo mouse model were utilized. Treatment of Caco-2 cells with TGF-[beta]1 (10 ng/ml, 60 min) stimulated SERT activity (~2 fold, P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0120447