SAOS-2 Osteosarcoma Cells Bind Fibroblasts via ICAM-1 and This Is Increased by Tumour Necrosis Factor-[alpha]

We recently reported exchange of membrane and cytoplasmic markers between SAOS-2 osteosarcoma cells and human gingival fibroblasts (h-GF) without comparable exchange of nuclear markers, while similar h-GF exchange was seen for melanoma and ovarian carcinoma cells. This process of "cellular sipp...

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Bibliographic Details
Published in:PloS one 2014-06, Vol.9 (6)
Main Authors: David, Manu S, Kelly, Elizabeth, Cheung, Ivan, Xaymardan, Munira, Moore, Malcolm A. S, Zoellner, Hans
Format: Article
Language:English
Subjects:
Osteosarcoma
Tumor necrosis factor
Tumors
Online Access:Get full text
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Summary:We recently reported exchange of membrane and cytoplasmic markers between SAOS-2 osteosarcoma cells and human gingival fibroblasts (h-GF) without comparable exchange of nuclear markers, while similar h-GF exchange was seen for melanoma and ovarian carcinoma cells. This process of "cellular sipping" changes phenotype such that cells sharing markers of both SAOS-2 and h-GF have morphology intermediate to that of either cell population cultured alone, evidencing increased tumour cell diversity without genetic change. TNF-[alpha] increases cellular sipping between h-GF and SAOS-2, and we here study binding of SAOS-2 to TNF-[alpha] treated h-GF to determine if increased cellular sipping can be accounted for by cytokine stimulated SAOS-2 binding. More SAOS-2 bound h-GF pe-seeded wells than culture plastic alone (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0101202