Evaluation of TNF-[alpha], IL-10 and IL-6 Cytokine Production and Their Correlation with Genotype Variants amongst Tuberculosis Patients and Their Household Contacts

• Published in: PloS one 2015-09, Vol.10 (9)
• Main Authors: Joshi, Lavanya, Ponnana, Meenakshi, Sivangala, Ramya, Chelluri, Lakshmi Kiran, Nallari, Prathiba, Penmetsa, Sitaramaraju, Valluri, Vijayalakshmi, Gaddam, Sumanlatha
• Format: Article
• Language: English
• Subjects: Adjustable rate mortgages; Care and treatment; Development and progression; Diagnosis; Genetic aspects; Patient outcomes; Tuberculosis; Tumor necrosis factor
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0137727

Household contacts of diagnostically established tuberculosis (TB) patients are highly susceptible to disease development. It is surmised that cytokines perhaps play a synergistic and a prognostic role in the activation of the otherwise latent infection in these house hold contacts. Evaluation of the cytokines and any of their inherent polymorphisms might provide a useful diagnostic tool in evaluating the immune regulation and the progression of the disease. The cytokines thus released in a paracrine manner in serum may also provide an indirect measure of the cytokine function. The present study was aimed to evaluate the levels of TNF-[alpha], IL-10 & IL-6 cytokines and their correlation with genotype variants amongst tuberculosis patients and their household contacts. The cytokine levels were estimated in serum by enzyme-linked immunosorbent assay (ELISA) and their polymorphisms were studied by amplification refractory mutation system polymerase chain reaction (ARMs PCR) in active pulmonary tuberculosis patients (APTB = 150), household contacts (HHC = 190), and healthy controls (HC = 150). The median values of TNF-[alpha] cytokine were significantly high among APTB and HHC compared to HCs (P< 0.0001 and 0.0001). IL-6 levels also were elevated among APTB compared to HHC and HC, and a significant difference was observed between APTB and HHC at P