Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery/Odreoivanje osmotskih osobina zivotinjskih eritrocita protocnom citometrijom u cilju njihovog inzenjeringa kao nosaca lekova

• Published in: Hemijska industrija 2015-01, p.67
• Main Authors: Kostic, Ivana T, Ilic, Vesna Lj, Bukara, Katarina M, Mojsilovic, Slavko B, Duric, Zorka Z, Draskovic, Petra, Bugarski, Branko M
• Format: Article
• Language: English
• Subjects: Chemical properties; Drug delivery systems; Drugs; Erythrocytes; Flow cytometry; Identification and classification; Methods; Observations; Osmosis; Technology application; Vehicles
• ISSN: 0367-598X
• DOI: 10.2298/HEMIND140124021K

Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to investigate the possibility of use the flow cytometry as a tool for determination the osmotic behaviour of porcine and bovine erythrocytes, and thus facilitates the engineering of erythrocytes from animal blood to be drug carriers. The method of flow cytometry successfully provided the information about bovine and porcine erythrocyte osmotic fragility, and made the initial steps in assessment of erythrocyte shape in a large number of erythrocytes. Although this method is not able to confirm the swelling of porcine erythrocytes, it indicated the differences in porcine erythrocytes that had basic hematological parameters inside and outside the reference values. In order to apply/use the porcine and bovine erythrocytes as drug carriers, the method of flow cytometry, confirming the presence of osmotically different fractions of red blood cells, indicated that various amounts of the encapsulated drug in porcine and bovine erythrocytes can be expected. Keywords: flow cytometry, osmotic swelling, osmotic fragility, mechanical fragility, microcytic anaemia, erythrocytes. Uprkos cinjenici da su metode koje se baziraju na osmotskim osobinama ce lija najcesce koriscene metode za inkapsulaciju lekova u humane i zivotinjske eritrocite, podaci o osmotskim osobinama eritrocita zivotinjskog porekla su vrlo oskudni. Cilj ovog rada bio je ispitivanje mogucnosti koriscenja metode protocne citometrije za odredivanje osmotskih osobina svinjskih i govedih eritrocita, cime bi se olaksao inzenjering pomenutih zivotinjskih eritrocita za otpustanje lekova. Metodom protocne citometrije uspesno su dobijene informacije o osmotskoj fragilnosti svinjskih i govedih eritrocita i nacinjeni su pocetni koraci u proceni oblika velikog broja eritrocita. lako ova metoda nije uspela da potvrdi bubrenje svinjskih eritrocita, ukazala je na razliku u uzorcima svinjskih eritrocita koji su imali osnovne hematoloske parametre izvan i unutar referentnih vrednosti. U cilju primene svinjskih i govedih eritrocita kao nosaca lekova, metoda protocne citometrije je, potvrdivsi prisustvo osmotski razlicitih frakcija eritrocita, ukazala na to da se razlicite kolicine inkapsuliranog leka u pojedinacnim, kako svinjskim, tako i