An enzyme-free electrochemical method for the determination of E. coli using [Fe.sub.3][O.sub.4] nanocomposites with a Si[O.sub.2] shell modified by ferrocene

An electrochemical immunoassay method is developed for determining the strain of Escherichia coli ATCC 25922 with the use of electroactive nanocomposite particles based on magnetite [Fe.sub.3][O.sub.4] coated by Si[O.sub.2] modified with ferrocene as a signal label. A sensitive, direct, and easily m...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2015-05, p.540
Main Authors: Kozitsina, A.N, Malysheva, N.N, Utepova, I.A, Glazyrina, Yu. A, Matern, A.I, Brainina, Kh.Z, Chupakhin, O.N
Format: Article
Language:English
Subjects:
Biological assay
Electrochemical analysis
Escherichia coli
Ferrocene
Identification and classification
Methods
Silica
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Summary:An electrochemical immunoassay method is developed for determining the strain of Escherichia coli ATCC 25922 with the use of electroactive nanocomposite particles based on magnetite [Fe.sub.3][O.sub.4] coated by Si[O.sub.2] modified with ferrocene as a signal label. A sensitive, direct, and easily measured analytical response is obtained by cyclic voltammetry. The developed method enables the determination of bacterial cells at a minimum concentration of 2.3 x [10.sup.3] CFU/mL. The analytical range is 2.3 x [10.sup.3]-2.3 x [10.sup.7] CFU/mL; the detection limit is 2.5 x [10.sup.2] CFU/mL. The required sample volume is 0.5 mL, and the analysis time is 40 min.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934815050068