Determination of negundoside and agnuside in Vitex negundo by qNMR and method validation

Negundoside and agnuside are reported as marker constituents for standardization of Vitex negundo (V. negundo) leaves. This is the first report on determination and quantification of negundoside and agnuside by qNMR in the leaves of V. negundo , collected from various regions of India. The developed...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2015-05, Vol.70 (5), p.639-646
Main Authors: Roy, Somendu Kumar, Bairwa, Khemraj, Grover, Jagdeep, Srivastava, Amit, Jachak, Sanjay Madhukar
Format: Article
Language:English
Subjects:
Analytical Chemistry
Chemical properties
Chemistry
Chemistry and Materials Science
Identification and classification
Lamiaceae
Methods
Nuclear magnetic resonance spectroscopy
Physiological aspects
Phytochemicals
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Summary:Negundoside and agnuside are reported as marker constituents for standardization of Vitex negundo (V. negundo) leaves. This is the first report on determination and quantification of negundoside and agnuside by qNMR in the leaves of V. negundo , collected from various regions of India. The developed qNMR method showed good linearity ( r 2 ≥ 0.9994), high precision (RSD < 5%) and a good recovery (99.79 and 101.35%) for both the compounds. Therefore, the developed qNMR method is accurate and reliable for the quality control of V. negundo . The qNMR method is useful in quality control of plant extracts without the need of extensive sample preparation and chromatographic separation.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934815050147