Relationship between Neubauer counting chamber and spectrophotometer used for the sperm concentration determination in collared peccaries

In ejaculates derived from 28 collared peccaries, we verified the existence of relationships between sperm concentration determined by the Neubauer counting chamber and the tramitance verified by spectrophotometer, under wavelengths varying from 530 to 590nm. Ejaculates presented a concentration of...

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Bibliographic Details
Published in:Ciência rural 2014-08, Vol.44 (8), p.1494
Main Authors: da Silva, Andreia Maria, Peixoto, Gislayne Christianne Xavier, Bezerra, Jose Artur Brilhante, Castelo, Thiberio de Souza, Santos, Erika Aparecida Araujo, Silva, Alexandre Rodrigues
Format: Article
Language:Spanish
Subjects:
Analytical instruments
Optical instruments
Sperm
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Summary:In ejaculates derived from 28 collared peccaries, we verified the existence of relationships between sperm concentration determined by the Neubauer counting chamber and the tramitance verified by spectrophotometer, under wavelengths varying from 530 to 590nm. Ejaculates presented a concentration of 283.9[+ or -]30.8x106sperm m-1, varying from 30 to 640x106sperm m[L.sup.-1]. Values for tramitance varied from 36.9 to 96.3, under different wavelengths. No significant relationship was verified between two methods (P>0.05). Thus, the spectrophotometer is not recommended for routine procedures of sperm concentration measurement in collared peccaries.
ISSN:0103-8478
DOI:10.1590/0103-8478cr20130928