Use of alternative media and different types of recipients in a laboratory culture of Ankistrodesmus gracilis em laboratorio

• Published in: Acta scientiarum. Biological sciences 2011-07, Vol.33 (3), p.247
• Main Authors: Sipauba-Tavares, Lucia Helena, Millan, Rodrigo Ney, Berchielli, Flavia de Almeida, Braga, Francisco Manoel de Souza
• Format: Article
• Language: Spanish
• ISSN: 1679-9283
• DOI: 10.4025/actascibiolsci.v33i3.8046

A laboratory culture of Ankistrodesmus gracilis algae was evaluated by studying the biology of the species and its chemical composition in a traditional medium ([CHU.sub.12]) and in two alternative culture media, NPK (20-5-20) and macrophyte (Eichhornia crassipes) + NPK, in three different types of recipients (fiberglass, carboy and plastic bag). First peak in the growth curve of Ankistrodesmus gracilis occurred on the ninth day in macrophyte + NPK medium (74.16 x [10.sup.5] cells [mL.sup.-1]) in a fiberglass recipient. However, highest density (p < 0.01) was reported in medium [CHU.sub.12] (122.87 x [10.sup.5] cells [mL.sup.-1]) in a plastic bag on the twelfth day. Cell density was over 70 x [10.sup.5] cells [mL.sup.-1] starting on the twelfth day. Growth rate of A. gracilis was similar (p > 0.05) in culture media in the three recipients. Protein and fiber were similar (p > 0.05) in the treatments, but lipids were higher (p < 0.05) in NPK. Nitrate, ammonia, total phosphorus and orthophosphate contents were over 1 mg [L.sup.-1] in NPK (p < 0.01). Results show that alternative media, such as NPK and macrophyte + NPK, are possible for large-scale culture of A. gracilis cultured in three types of recipients. Costs are low, occupying less space when cultured in plastic bags and in the laboratory. Keywords: algae, macrophyte medium, NPK, [CHU.sub.12], biochemical composition, growth. Utilizacao de meios alternativos e diferentes tipos de recipientes no cultivo de Ankistrodesmus gracilis (Reinsch) Korshikov (Chlorophyceae) em laboratorio. O objetivo do estudo foi avaliar os aspectos biologicos e a composicao quimica da alga Ankistrodesmus gracilis em laboratorio utilizando um meio tradicional ([CHU.sub.12]) e dois meios alternativos, NPK (20-5-20) e macrofita (Eichhornia crassipes) + NPK em tres diferentes tipos de recipientes (cuba de fibra de vidro translucido, garrafoes e saco plastico). O primeiro pico de densidade celular de Ankistrodesmus gracilis ocorreu no nono dia da curva de crescimento em meio macrofita+NPK (74,16 x [10.sup.5] celulas [mL.sup.-1]) no recipiente de fibra de vidro, porem a maior densidade (p < 0,01) foi observada no meio [CHU.sub.12] (122,87 x [10.sup.5] celulas [mL.sup.-1]) em saco plastico no decimo segundo dia, a partir do qual a densidade celular permaneceu acima de 70 x [10.sup.5] celulas [mL.sup.-1]. A taxa de crescimento de A. gracilis foi similar (p > 0,05) nos tres recipientes e meios de cultivo. Os teores de proteina e fibra f