Impact of a CXCL12/CXCR4 Antagonist in Bleomycin Induced Hepatic Fibrosis in Mice

• Published in: PloS one 2016-03, Vol.11 (3)
• Main Authors: Chow, Leola N, Schreiner, Petra, Ng, Betina Y. Y, Lo, Bernard, Hughes, Michael R, Scott, R. Wilder, Gusti, Vionarica, Lecour, Samantha, Simonson, Eric, Manisali, Irina, Barta, Ingrid, McNagny, Kelly M, Crawford, Jason, Webb, Murray, Underhill, T. Michael
• Format: Article
• Language: English
• Subjects: Analysis; Care and treatment; Chemokines; Liver diseases; Pulmonary fibrosis
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0151765

Modulation of chemokine CXCL12 and its receptor CXCR4 has been implicated in attenuation of bleomycin (BLM)-induced pulmonary fibrosis and carbon tetrachloride (CCl.sub.4 )-induced hepatic injury. In pulmonary fibrosis, published reports suggest that collagen production in the injured lung is derived from fibrocytes recruited from the circulation in response to release of pulmonary CXCL12. Conversely, in hepatic fibrosis, resident hepatic stellate cells (HSC), the key cell type in progression of fibrosis, upregulate CXCR4 expression in response to activation. Further, CXCL12 induces HSC proliferation and subsequent production of collagen I. In the current study, we evaluated AMD070, an orally bioavailable inhibitor of CXCL12/CXCR4 in alleviating BLM-induced pulmonary and CCl.sub.4 -induced hepatic fibrosis in mice. Similar to other CXCR4 antagonists, treatment with AMD070 significantly increased leukocyte mobilization. However, in these two models of fibrosis, AMD070 had a negligible impact on extracellular matrix deposition. Interestingly, our results indicated that CXCL12/CXCR4 signaling has a role in improving mortality associated with BLM induced pulmonary injury, likely through dampening an early inflammatory response and/or vascular leakage. Together, these findings indicate that the CXCL12-CXCR4 signaling axis is not an effective target for reducing fibrosis.