Loading…
In vitro establishment of Comanthera curralensis, "sempre viva" native of Chapada Diamantina--Bahia/Estabelecimento in vitro de Comanthera curralensis, "sempre viva", nativa da Chapada Diamantina--Bahia
The goal of the present study was to evaluate the germination, initial growth, and in vitro co-cultivation of Comanthera curralensis Moldenke, a "sempre viva" native of the Chapada Diamantina state of Bahia. Full strength (MS) and half-strength MS (MS 1/2) growth media supplemented with tw...
Saved in:
Published in: | Ciência rural 2016-06, Vol.46 (6), p.991 |
---|---|
Main Authors: | , , , , |
Format: | Article |
Language: | English |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | The goal of the present study was to evaluate the germination, initial growth, and in vitro co-cultivation of Comanthera curralensis Moldenke, a "sempre viva" native of the Chapada Diamantina state of Bahia. Full strength (MS) and half-strength MS (MS 1/2) growth media supplemented with two different sucrose concentrations (15 and 30g [L.sup.-1]) were tested for germination and initial plant growth. Three different plant densities were tested by in vitro culture (8, 10 and 12 plants per container). MS 1/2 medium with 15g [L.sup.-1] sucrose resulted in a higher percentage of germination and plant growth for the in vitro establishment of C. curralensis. The use of 12 plants per container is indicated for cost reduction in C. curralensis in vitro production. Key words: Eriocaulaceae, tissue culture, germination, in vitro growth, co-cultivation. Este trabalho teve como objetivo avaliar a germinacao, o crescimento inicial e o co-cultivo in vitro de Comanthera curralensis Moldenke, uma "sempre viva" nativa da Chapada Diamantina-BA. Para germinacao e crescimento inicial, foram testados os meios de cultura MS completo e MS' suplementados com duas concentragoes de sacarose (15 e 30g[L.sup.-1]); no cultivo in vitro, foram testadas tres quantidades de plantas por recipiente (8,10 e 12). A utilizacao do meio MSA com 15g[L.sup.-1] de sacarose proporcionou maiores porcentagem de germinacao crescimento das plantas no estabelecimento in vitro de C. curralensis, e o uso de 12 plantas por recipiente e indicado para a reducao de custos na producao in vitro da especie. Palavras-chave: Eriocaulaceae, cultura de tecidos, germinacao, crescimento in vitro, co-cultivo. |
---|---|
ISSN: | 0103-8478 |
DOI: | 10.1590/0103-8478cr20131091 |