Pollen grain viability of coconut accessions at low temperatures/Viabilidade de graos de polen de acessos de coqueiro em baixas temperaturas

• Published in: Acta scientiarum. Agronomy 2014-04, Vol.36 (2), p.227
• Main Authors: Machado, Caroline de Araujo, Moura, Catrine Regina Feitosa, de Lemos, Eurico Eduardo Pinto, Ramos, Semiramis Rabelo Ramalho, Ribeiro, Francisco Elias, Ledo, Ana da Silva
• Format: Article
• Language: Spanish
• ISSN: 1679-9275
• DOI: 10.4025/actasciagron.v36i2.17346

The aim of this study was to evaluate the storage potential of the pollen grains of Brazil Green Dwarf (BGD), Brazilian Tall (BRA) and Cameroon Red Dwarf (CRD) coconut accessions under different storage conditions. Representative plants of BGD, BRA and CRD accessions were selected from the Coconut Active Germplasm Bank of Embrapa Coastal Tablelands. The pollen grains were collected, placed in cryotubes and maintained under the following storage conditions: T1, refrigerator (-4°C); T2, freezer (-20°C); T3, ultrafreezer (- 80°C) and T4, liquid nitrogen (-196°C). The pollen grain viability was determined by staining with 1% acetic carmine and in vitro germination in a Lora culture medium at 30 and 60 days after the storage. Storage under refrigerator (-4°C), freezer (-80°C) and liquid nitrogen (-196°C) conditions promoted a better pollen grain viability of the Brazilian Tall coconut accession. Storage under freezer (-20°C) and liquid nitrogen (- 196°C) conditions promoted a better pollen grain viability of the Cameroon Red Dwarf coconut accession. Storage under freezer (-20°C and -80°C) and liquid nitrogen (-196°C) conditions promoted a better pollen grain viability of the Brazil Green Dwarf coconut accession. T he pollen grain viability of the Brazilian Tall coconut accession is stable after 30 and 60 days of storage. Keywords: Cocos nucifera L., genetic resources, conservation, meio de cultura, germinacao in vitro. O objetivo deste estudo foi avaliar o potencial de armazenamento de graos de polen de acessos de coqueiro Anao Verde do Brasil de Jiqui (AVeBrJ), coqueiro Gigante do Brasil Praia do Forte (GBrPF) e coqueiro Anao Vermelho dos Camaroes (AVC) em diferentes condicoes de armazenamento. Plantas matrizes dos acessos foram selecionados no Banco Ativo de Germoplasma de Coco da Embrapa Tabuleiros Costeiros. Os graos de polen foram coletados, armazenados em criotubos e mantidos nas seguintes condicoes de armazenamento: T1: Congelador (-4°C) T2: Freezer (-20°C), T3: Freezer (-80°C) e T4: Nitrogenio liquido (- 196°C). A viabilidade graos de polen foi determinada por coloracao com carmim acetico a 1% e por germinacao in vitro em meio de cultura Lora aos 30 e 60 dias apos o armazenamento. O armazenamento em refrigeracao (-4°C), freezer (- 80°C) e nitrogenio liquido (-196°C) promoveu uma melhor viabilidade de graos de polen para o acesso GBrPF. O armazenamento em freezer (-20°C) e nitrogenio liquido (-196°C) proporcionou melhor viabilidade de graos de polen para