Estimation of genetic distance among genotypes of caraway por RAPD-PCR

• Published in: Acta scientiarum. Agronomy 2014-04, Vol.36 (2), p.183
• Main Authors: Seidler-Lozykowska, Katarzyna, Kuczynska, Anetta, Mikolajczyk, Katarzyna, Nowakowska, Joanna, Bocianowski, Jan
• Format: Article
• Language: Spanish
• ISSN: 1679-9275
• DOI: 10.4025/actasciagron.v36i2.18197

In order to estimate genetic diversity among starting materials and breeding strains of caraway, a collection of 17 accessions from botanical gardens in Europe, two cultivars 'Rekord' and 'Konczewicki', and four own breeding strains were analyzed by RAPD-PCR. The representative samples, each of five individual plants of accession, cultivar or strain, were taken from young rosette leaves. Forty of Genset Oligos RAPD primers were used for analysis and eight of them produced clear and reproducible banding patterns. In total, 62 banding patterns were obtained revealing 23 polymorphic bands, whereas the number of polymorphic bands ranged from two to four for one primer. The GS12 and GS43 primers generated two polymorphic bands, while each of the GS8, GS21, GS22, GS41 and GS53 primers generated three bands. The GS53 primer was the most informative one, revealing 60% of the estimated polymorphism. The estimated value of genetic distance ranged from 0.22 to 0.67. The lowest genetic distance was found between accessions from Cluj and Lousanne (0.22). The highest genetic distance was estimated between accession from Berlin and the strain no. 6 of cultivar 'Konczewicki' (0.67). UPGMA cluster analysis, based on eight RAPD primers, categorized the analyzed genotypes into four groups. Keywords: Carum carvi L., genetic distance, genotype, RAPD. Dezessete acessos oriundos de hortos botanicos na Europa, dois cultivares Rekord e Konczewicki, e quatro cepas foram analisados por RAPD-PCR para determinar a diversidade genetica entre os materiais incipientes e as cepas do cominho. As amostras com cinco plantas individuais de acessos, cultivares ou cepas derivaram-se de jovens folhas em roseta. Quarenta Genset Oligos RAPD primers foram usados para analise, dos quais oito produziram padroes claros e reproduziveis de banda. Sessenta e dois padroes de bandas foram obtidos, revelando 23 bandas polimorficas, enquanto o numero de bandas polimorficas chegou entre dois a quatro para um primer. Os primers GS12 e GS43 prodiziram duas bandas polimorficas, enquanto cada um dos primers GS8, GS21, GS22, GS41 and GS53 gerou tres bandas. O primer mais informativo foi GS53, revelando 60% do polimorfismo estimado. A distancia genetica estava entre 0,22 e 0,67. A menor distancia genetica encontrou-se entre os acessos oriundos de Cluj e Lousanne (0,22) e a maior distancia genetica foi estimada entre o acesso oriundo de Berlin e a cepa 6 do cultivar 'Konczewicki' (0,67). A analise de agrupamento UPG