Magnetic Resonance Detection of CD34.sup.+ Cells from Umbilical Cord Blood Using a .sup.19F Label

Impaired homing and delayed recovery upon hematopoietic stem cell transplantation (HSCT) with hematopoietic stem cells (HSC) derived from umbilical cord blood (UCB) is a major problem. Tracking transplanted cells in vivo will be helpful to detect impaired homing at an early stage and allows early in...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2015-09, Vol.10 (9), p.e0138572
Main Authors: Duinhouwer, Lucia E, van Rossum, Bernard J. M, van Tiel, Sandra T, van der Werf, Ramon M, Doeswijk, Gabriela N, Haeck, Joost C, Rombouts, Elwin W. J. C, ter Borg, Mariëtte N. D, Kotek, Gyula, Braakman, Eric, Cornelissen, Jan J, Bernsen, Monique R
Format: Article
Language:English
Subjects:
Energy efficient buildings
Hematopoietic stem cell transplantation
Hematopoietic stem cells
Nuclear magnetic resonance spectroscopy
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Impaired homing and delayed recovery upon hematopoietic stem cell transplantation (HSCT) with hematopoietic stem cells (HSC) derived from umbilical cord blood (UCB) is a major problem. Tracking transplanted cells in vivo will be helpful to detect impaired homing at an early stage and allows early interventions to improve engraftment and outcome after transplantation. In this study, we show sufficient intracellular labeling of UCB-derived CD34.sup.+ cells, with .sup.19 F-containing PLGA nanoparticles which were detectable with both flow cytometry and magnetic resonance spectroscopy (MRS). In addition, labeled CD34.sup.+ cells maintain their capacity to proliferate and differentiate, which is pivotal for successful engraftment after transplantation in vivo. These results set the stage for in vivo tracking experiments, through which the homing efficiency of transplanted cells can be studied.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0138572