Aberrant Activation of the WNT/[beta]-Catenin Signaling Pathway in Lupus Nephritis

The canonical WNT pathway has been implicated as playing important roles in the pathogenesis of a variety of kidney diseases. Recently, WNT pathway activity was reported to be elevated in the renal tissue of a lupus mouse model. This study aimed to evaluate the potential role of the WNT pathway in t...

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Bibliographic Details
Published in:PloS one 2014-01, Vol.9 (1), p.e84852
Main Authors: Wang, Xiao-dong, Huang, Xin-fang, Yan, Qing-ran, Bao, Chun-de
Format: Article
Language:English
Subjects:
Analysis
Antibodies
Immunohistochemistry
Lupus
Nephritis
RNA
Online Access:Get full text
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Summary:The canonical WNT pathway has been implicated as playing important roles in the pathogenesis of a variety of kidney diseases. Recently, WNT pathway activity was reported to be elevated in the renal tissue of a lupus mouse model. This study aimed to evaluate the potential role of the WNT pathway in the pathogenesis of human lupus nephritis. The expression of [beta]-catenin was evaluated in renal biopsy specimens from lupus nephritis patients and control kidney tissues by immunohistochemistry and western blotting. Real-time polymerase chain reaction (RT-PCR) was used to detect RNA expression of [beta]-catenin, Dkk-1 and Axin2. Plasma concentrations of Dkk-1 were measured by ELISA. Immunohistochemistry and western blotting revealed increased expression of [beta]-catenin in the kidneys of patients with lupus nephritis compared with control kidney tissues (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0084852