Meloidogyne and Pratylenchus species in sugarcane fields in the state of Alagoas, Brazil/Especies de Meloidogyne e de Pratylenchus em areas cultivadas com cana-de-acucar no estado de Alagoas

• Published in: Ciência rural 2017-02, Vol.47 (2)
• Main Authors: Noronha, Marissonia de Araujo, Muniz, Maria de Fatima Silva, Cruz, Marcelo de Menezes, Assuncao, Mayara Castro, da Cunha, Jose Mauro Castro, de Oliveira, Ellen Rebecca Lopes, Miranda, Camila Goncalves dos Santos, Machado, Andressa Cristina Zamboni
• Format: Article
• Language: English
• ISSN: 0103-8478
• DOI: 10.1590/0103-8478cr20151402

The objective of this study was to accomplish a survey on populations of Meloidogyne and Pratylenchus species in sugarcane farming areas in the state of Alagoas, Brazil. Twenty samples of soil and roots were processed to extract and quantify nematodes; however, the identification of Meloidogyne species was performed using only 12 samples. Pratylenchus spp. were reported at moderate population levels of 68-1556 specimens 50[g.sup.-1] of roots and 2-298 specimens 100[cm.sup.-3] of soil in twenty analyzed samples. For Meloidogyne spp., these values were of 12-487 specimens 50[g.sup.-1] of roots and 0-140 specimens 100[cm.sup.-3] of soil. Based on electrophoresis of esterase isozymes, M. incognita was reported to be the most frequent species, followed by M. javanica and M. arenaria. Pratylenchus species identified through morphometrical and morphological characteristics were P. zeae and P. brachyurus, with predominance for the first species. No significant correlation (P[less than or equal to]0.05) were reported between nematode populations and sugarcane cropping systems. Key words: field survey, plant-parasitic nematodes, Saccharum spp. O objetivo deste trabalho foi realizar um levantamento de especies de Meloidogyne e de Pratylenchus existentes em areas de cultivo de cana-de-acucar, no estado de Alagoas. Vinte amostras de solo e raizes foram processadas para a extracao e quantificacao, sendo a identificacao das especies de Meloidogyne realizada em 12 amostras. Detectou-se a presenca de Pratylenchus spp. em niveis populacionais medios de 68-1556 especimes 50[g.sup.-1] de raizes e 2-298 100[cm.sup.-3] de solo, em todas as amostras. Para Meloidogyne spp. , esses valores foram de 12-487 50[g.sup.-1] de raizes e 0-140 100[cm.sup.-3] de solo. Com base na eletroforese da isoenzima esterase, M. incognita foi a especie mais frequente, seguida por M. javanica e M. arenaria. As especies de Pratylenchus identificadas pelas caracteristicas morfologicas e morfometricas, foram P. zeae e P. brachyurus, com predominio da primeira especie. Nao houve correlacoes significativas (P