Performance evaluation of BD FACSPresto.sup.[TM] point of care CD4 analyzer to enumerate CD4 counts for monitoring HIV infected individuals in Nigeria

• Published in: PloS one 2017-05, Vol.12 (5), p.e0178037
• Main Authors: Negedu-Momoh, Olubunmi Ruth, Jegede, Feyisayo Ebenezer, Yakubu, Ali, Balogun, Oluseyi, Abdullahi, Musa, Badru, Titilope, Oladele, Edward Adekola, Agbakwuru, Chinedu, Khamofu, Hadiza, Torpey, Kwasi
• Format: Article
• Language: English
• Subjects: CD4 lymphocytes; Diagnosis; Drug therapy; Highly active antiretroviral therapy; HIV infections; Measurement; Physiological aspects
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0178037

Despite the upsurge in support and intervention of donor agencies in HIV care and treatment programing in Sub-Sahara African, antiretroviral (ART) programs are still confronted with access and coverage challenges which influence enrolment of new patients. This study investigated the validity of point of care BD FACSPresto.sup.[TM] CD4 analyzer for CD4+ cell count, overall agreement, correlation, sensitivity, and specificity in comparison to a reference standard flow cytometry method. We also assessed the feasibility of use among non-laboratorians. Blood samples from 300 HIV infected individuals were analyzed for CD4+ T cell and CD4%, using finger prick capillary sample from 150 PMTCT clients and 150 ART clients at Murtala Mohammed Specialist Hospital, Kano, Nigeria. Their venous samples were compared on a flow cytometry reference method using BD FACSCount CD4+ count system. The accuracy of the BD FACSPresto machine in comparison to BD FACSCount was evaluated. Statistical analysis was carried out using STATA (version 12). Bland-Altman method and correlation analysis were used to analyze agreement between both measurements. In addition, sensitivity and specificity of both measurements were determined. Statistical significance was set at p-value