Environmental DNA assays for the sister taxa sauger

Sauger (Sander canadensis) and walleye (S. vitreus) are percid fishes that naturally co-occur throughout much of the eastern United States. The native range of sauger extends into the upper Missouri River drainage where walleye did not historically occur, but have been stocked as a sport fish. Sauge...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2017-04, Vol.12 (4), p.e0176459
Main Authors: Dysthe, Joseph C, Carim, Kellie J, Ruggles, Michael, McKelvey, Kevin S, Young, Michael K, Schwartz, Michael K
Format: Article
Language:English
Subjects:
Analysis
Biochemical assays
DNA
Fishes
Genetic aspects
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Sauger (Sander canadensis) and walleye (S. vitreus) are percid fishes that naturally co-occur throughout much of the eastern United States. The native range of sauger extends into the upper Missouri River drainage where walleye did not historically occur, but have been stocked as a sport fish. Sauger populations have been declining due to habitat loss, fragmentation, and competition with non-native species, such as walleye. To effectively manage sauger populations, it is necessary to identify areas where sauger occur, and particularly where they co-occur with walleye. We developed quantitative PCR assays that can detect sauger and walleye DNA in filtered water samples. Each assay efficiently detected low quantities of target DNA and failed to detect DNA of non-target species with which they commonly co-occur.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0176459