Processed fruit juice ready to drink: screening acute toxicity at the cellular level/Sucos de frutas industrializados prontos para consumo: screening da toxicidade aguda em nivel celular

• Published in: Acta scientiarum. Biological sciences 2017-04, Vol.39 (2), p.195
• Main Authors: da Silva, Erick Leal, Sales, Ila Monize Sousa, dos Santos, Fabelina Karollyne Silva, Peron, Ana Paula
• Format: Article
• Language: Spanish
• ISSN: 1679-9283
• DOI: 10.4025/actascibiolsci.v39i2.33699

The present study evaluated the acute toxicity at the cellular level of processed juice ready for consumption Orange and Grape flavors, produced by five companies with significant influence on the food market of South American countries, especially in Brazil. This evaluation was performed in root meristem cells of Allium cepa L., at the exposure times of 24 and 48 hours, directly with marketed liquid preparations. Based on the results, it was found that fruit juices, of all companies considered, promoted significant antiproliferative effect to root meristems at the exposure time of 24 hours and resulted in at both exposure times, statistically significant number of mitotic spindle changes and chromosomal breaks. Therefore, under the study conditions, all juice samples analyzed were cytotoxic, genotoxic and mutagenic to root meristem cells. These results indicate that such beverages have relevant potential to cause cellular disorders and, thus, need to be evaluated more fully in more complex test systems, as those in rodents, and then establish specific toxicity at the cellular level of these juices and ensure the well-being of those who consume them. Keywords: industrialized juice, cytotoxicity, genotoxicity, mutagenicity, meristem tissue. Objetivou-se neste trabalho avaliar a toxicidade aguda em nivel celular de sucos industrializados prontos para beber, sabor laranja e uva, de cinco empresas alimenticias de reconhecida reputacao no mercado de alimentos em paises da America do Sul, especialmente o Brasil. Esta avaliacao se deu por meio das celulas meristematicas de raizes de Allium cepa L., nos tempos de exposicao 24 e 48h, diretamente nos preparados liquidos comercializados. Com base nos resultados obtidos verificou-se que os sucos de frutas, de todas as empresas consideradas, promoveram expressivo efeito antiproliferativo aos meristemas de raizes ja no tempo de exposicao 24h, e ocasionaram numero estatisticamente significativo de alteragoes de fuso mitotico e quebras cromossomicas nas celulas do tecido analisado em todo o tempo de analise. Portanto, nas condigoes de estudo estabelecidas, os sucos das empresas avaliadas foram citotoxicos, genotoxicos e mutagenicos. Estes resultados sao importantes em razao de indicarem que tais alimentos tem relevante potencial em causar disturbios celulares e, portanto, devem ser avaliados em sistemas com testes mais complexo, como os em roedores, para, dessa forma, estabelecer com propriedade a toxicidade em nivel celu