Loading…

Zymomonas mobilis immobilized on loofa sponge: levan and ethanol production in semi-continuous fermentation/Zymomonas mobilis imobilizada em bucha vegetal: producao de etanol e levana por sistema semi-continuo

Zymomonas mobilis is a promising microorganism in the biotechnological production of ethanol and levan due to its low biomass and high tolerance to ethanol concentrations. Ethanol and levan production by Z. mobilis CDBB-603 immobilized on loofa sponge using semi-continuous fermentation is evaluated....

Full description

Saved in:
Bibliographic Details
Published in:Acta scientiarum. Technology 2017-04, Vol.39 (2), p.135
Main Authors: Santos, Vidiany Aparecida Queiroz, Vega-Estrada, Jesus, Horcasitas, Maria Del Carmen Montes, Garcia-Cruz, Crispin Humberto
Format: Article
Language:Spanish
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Zymomonas mobilis is a promising microorganism in the biotechnological production of ethanol and levan due to its low biomass and high tolerance to ethanol concentrations. Ethanol and levan production by Z. mobilis CDBB-603 immobilized on loofa sponge using semi-continuous fermentation is evaluated. The first experiment, conducted with initial volume 50 mL and incubated for 24 hours, produced maximum ethanol and levan production, respectively 13.56 g [L.sup.-1] and 23.94 g [L.sup.-1], with 250 g [L.sup.-1] sucrose and without agitation, at 30[degrees]C. The second experiment was based on the best condition obtained in the first, using fermenter with production scale of 200 mL and by semi-continuous process. The second experiment also assessed immobilized biomass reuse during 10 days (240 hours) and produced higher ethanol (34.64 g [L.sup.-1]) and levan (26.40 g [L.sup.-1]) production rates than in the first experiment. Experiment 2 also verified that the microorganism remained viable and produced ethanol and levan until the last (10th) recycle day. Keywords: Luffa cylindrical, attachment, exopolysaccharide, alcoholic fermentation. Zymomonas mobilis e um microrganismo com alto potencial tecnologico para a producao de etanol e levana, devido a baixa formacao de biomassa e alta tolerancia a elevadas concentracoes de etanol. O objetivo deste estudo foi avaliar a producao de etanol e levana por Z. mobilis imobilizada em bucha vegetal, utilizando fermentacao semi-continua. No primeiro experimento, conduzido com volume inicial de 50 mL de meio de cultivo, obteve-se maxima producao de etanol (13.56 g [L.sup.-1]) e levana (23.94 g [L.sup.-1]), utilizando 250 g [L.sup.-1] de sacarose como substrato, ausencia de agitacao e temperatura de 30[degrees]C durante 24h. O segundo experimento, foi realizado com base nas melhores condicoes de fermentacao obtidas no primeiro, utilizando processo semi-continuo em reator de bancada e 200 mL de volume final. No segundo experimento, foi avaliada a reutilizacao do suporte contendo o microrganismo imobilizado durante dez dias (240h) de fermentacao. O experimento 2 apresentou maior producao de etanol (34.64 g [L.sup.-1]) e levana (26.40 g [L.sup.-1]) em relacao ao primeiro experimento e o microrganismo imobilizado permaneceu viavel e produziu etanol e levana ate o ultimo dia da fermentacao semicontinua (10[degrees] reciclo). Palavras-chave: Luffa cylindrica, adsorcao, exopolissacarideo, fermentacao alcoolica.
ISSN:1806-2563
DOI:10.4025/actascitechnol.v39i2.27625