The prozone effect exerted by the complement-binding anti-Le a on anti-D

Prozone phenomenon is seen with very high-titer antibodies in an immune serum. The prozone effect on anti-D by a low-titer anti-Le was investigated associated with neonatal jaundice. Standard methods were used in investigations. The child was born at full-term developed mild jaundice. With weak dire...

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Bibliographic Details
Published in:Asian journal of transfusion science 2017-07, Vol.11 (2), p.188, Article 188
Main Authors: Joshi, Sanmukh R, Parekh, Kamlesh H
Format: Article
Language:English
Subjects:
Antibodies
Bilirubin
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Summary:Prozone phenomenon is seen with very high-titer antibodies in an immune serum. The prozone effect on anti-D by a low-titer anti-Le was investigated associated with neonatal jaundice. Standard methods were used in investigations. The child was born at full-term developed mild jaundice. With weak direct antiglobulin test+, her indirect serum bilirubin was progressed to 27.5 mg/dL in 48 h. Anti-D and anti-Le were detected in the mother. Both these antibodies were detected in the child's serum though the eluate from red blood cells (RBCs) contained only anti-D. Mother's anti-D was masked by anti-Le if the RBCs possessed both the antigens together. Anti-D was revealed only with D-positive RBCs lacking Le or if the serum was modified by mixing with Le saliva or was heated at 56°C or fortified with citrate solution. An anti-D showed prozone effect exerted by the complement-fixing anti-Le in the test.
ISSN:0973-6247
1998-3565
DOI:10.4103/0973-6247.214328