Topically administered rhGM-CSF affects PPAR[beta] expression in the stasis zone

• Published in: Experimental and therapeutic medicine 2017-11, Vol.14 (5), p.4825
• Main Authors: Fu, Gen-Feng, Tian, She-Min, Cha, Xin-Jian, Huang, Hong-Jun, Lou, Ji-He, Wei, Ying, Xia, Cheng-De, Li, Yong-Lin, Niu, Xi-Hua
• Format: Article
• Language: English
• Subjects: Burns; Care and treatment; Genetic aspects; Macrophage colony stimulating factor; Physiological aspects; Wound healing
• ISSN: 1792-0981
• DOI: 10.3892/etm.2017.5146

Using a rat comb thermal damage model, we investigated the effects of topically administered recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) on peroxisome proliferator-activated receptor PPAR[beta] expression. We created bilateral comb scald models on the backs of fifty Sprague-Dawley rats. The left sides of the backs served as the experimental group and the right sides served as the control group. The experimental group received topically applied rhGM-CSF hydrogel and the control group did not. The survival situations of the stasis zones were compared between the experimental and control groups on the 1st, 3rd, 7th, 14th and 21st post-burn days. Tissues from the surviving stasis zones of both groups were collected at different time-points. Reverse transcriptase-polymerase chain reaction (RT-PCR) and western blotting were used to detect the PPAR[beta] mRNA and protein expression levels. Immunohistochemical methods were applied to detect the localization of PPAR[beta] protein expression. The results showed that, first, the tissue viability numbers for the stasis zones of the experimental group were significantly increased compared with those of the control group. Second, RT-PCR revealed that the PPAR[beta] mRNA expression first increased and then gradually declined in both groups. At all time-points, the expression level in the experimental group was increased compared with that in the control group and the highest expression levels were observed in both groups on the 3rd post-burn day. Third, western blot analysis revealed that the PPAR[beta] protein expression in both groups increased after thermal damage and then gradually decreased. PPAR[beta] protein expression in the experimental group was greater than that in the control group, and the highest expression quantities in both groups were observed on the 3rd post-burn day. In conclusion, rhGM-CSF hydrogel effectively promotes the expression of PPAR[beta], and the hydrogel had a specific protective effect for the stasis zone. Key words: rhGM-CSF, the stasis zone, PPAR[beta]