Misidentified Candida lusitaniae, Causative Agent of Fungemia, as Candida famata by Automated Identification System in a Patient With Immune Thrombocytopenia/Otomatize Idantifikasyon Sisteminin Immun Trombositopenili Bir Hastadaki Fungemi Etkeni Candida lusitaniae'yi Candida famata Olarak Hatali Bicimde Tanimlamasi

• Published in: KLIMIK dergisi 2017-12, Vol.30 (3), p.149
• Main Authors: Yapici, Oktay, Er, Halil, Eres-Saritas, Zubeyde, Ozhak-Baysan, Betil, Ogunc, Meral Dilara, Ongut, Gozde, Merdin, Alparslan
• Format: Article
• Language: English
• Subjects: Care and treatment; Case studies; Diagnosis; Mycoses; Septicemia
• ISSN: 1301-143X
• DOI: 10.5152/kd.2017.36

Since Candida species have different antifungal resistance characteristics, accurate identification of non-albicans Candida besides C. albicans is important for treatment of Candida infections with high mortality. In this report, a case of fungemia due to C. lusitaniae an opportunist pathogen for immunosuppressed patients, misidentified as C. famata by VITEK[R] 2 (bioMerieux, Marcy l'Etoile, France) automated identification system was presented. Since isolate was identified as C. lusitaniae by conventional methods, its identification was confirmed using both MALDI-TOF/MS (Bruker Daltonics, Bremen, Germany) and Phoenix 100 (Becton Dickinson, Sparks, MD, USA) automated system. This case demonstrated that even though most of the clinically important Candida species can be identified by conventional methods, automated systems do not always make accurate identifications for rare species in particular. For this reason, conventional diagnostic methods should also be used together with automated systems, and further molecular diagnostic methods should be applied if necessary. Klimik Dergisi 2017; 30(3): 149-52. Key Words: Candida lusitaniae, Candida famata, identification, fungemia. Candida turleri arasinda antifungal direnc farkliliklari goruldugunden, C. albicans'in yani sira albicans disi turlerin dogru bicimde tanimlanmasi, mortalitesi yuksek olan Candida infeksiyonlarinin tedavisi icin onemlidir. Bu yazida, immunosuprese hastalar icin firsatci patojen olan C. lusitaniae'nin, VITEK[R] 2 (bioMerieux, Marcy l'Etoile, Fransa) otomatize idantifikasyon sistemiyle hatali bicimde C. famata olarak tanimlandigi bir fungemi olgusu sunulmustur. Izolatin konvansiyonel yontemlerle C. lusitaniae olarak adlandirilmasi uzerine, tanimlama gerek MALDI-TOF/MS (Bruker Daltonics, Bremen, Almanya) gerekse Phoenix 100 (Becton Dickinson, Sparks, MD, ABD) otomatize sistemi kullanilarak dogrulanmistir. Bu olgu da gostermektedir ki klinik onemi olan Candida turlerinin cogunun konvansiyonel yontemlerle tanimlanabilmesine karsilik, otomatize sistemlerle, ozellikle nadir gorulen turler her zaman dogru bicimde tanimlanamayabilir. Bu nedenle tanimlamada otomatize sistemlerle birlikte konvansiyonel tani yontemleri de kullanilmali, gerektiginde daha ileri molekuler tani yontemlerine basvurulmalidir. Klimik Dergisi 2017; 30(3): 149-52. Anahtar Sozcukler: Candida lusitaniae, Candida famata, idantifikasyon, fungemi.