Functional Effects of Alagebrium Fonksiyonel Etkileri

• Published in: The Eurasian journal of medicine 2017-10, Vol.49 (3), p.188
• Main Authors: Toprak, Cigdem, Sirmagul, Basar, Yigitaslan, Semra
• Format: Article
• Language: English
• Subjects: Analysis; Cardiovascular physiology; Carotid arteries; Health aspects; Nitric oxide
• ISSN: 1308-8734
• DOI: 10.5152/eurasianjmed.2017.17046

Objective: In our study, the effects of glycosylated protein cross-link breaker, alagebrium was investigated on isolated rat carotid artery using myography Alagebrium showed vasodilator effect on carotid artery rings; particularly this effect was significantly increased in endothelium-intact rings. Materials and Methods: To clarify the vasodilator mechanism of alagebrium, different antagonists such as N(G)-Nitro-L-arginine methyl ester (L-NAME), glibenclamide, indomethacin, metoprolol, propranolol, tetraethylammonium, and calcium channel activator BAYK-8644 were used to reverse this effect. Results: Relaxation% responses to alagebrium were more significantly increased in intact endothelium than in denuded arteries. Blocking vasodilation related to channels (K-ATP PG12, BKca) and receptors ([beta]31, [beta]32) did not reverse the relaxation response to alagebrium. Vasodilator response to alagebrium was only slightly decreased after L-NAME incubation and significantly decreased after BAYK-8644 incubation. Conclusion: Results of present study suggest that the mechanism of alagebrium-induced vasodilator effect may include the blockage of L-type calcium channels and partially of the nitric oxide synthase enzyme. Keywords: Alagebrium, calcium channel blockage, carotid artery nitric oxide, vasodilatation Amac: Calismamizda, glikozile protein capraz bag kiricisi olan alagebriumun sicanlardan elde edilen karotis arter preparatlari uzerindeki etkisi myograf yontemi ile arastirilmistir. Alagebrium karotis arter preparat-larinda vazodilator etki gostermis ve bu etkinin ozellikle endoteli saglam damarlarda arttigi gozlenmistir Gerec ve Yontem: Alagebriumun vazodilator mekanizmasini aydinlatmak amaciyla N(G)-Nitro-L-arginine methyl ester (L-NAME), glibenklamid, indometazin, metoprolol, propranolol, tetraetilamonyum gibi antagonistler ve kalsiyum kanal aktivatoru olan BAYK 8644 bu etkiyi geri cevirmek amaciyla kullanilmistir Bulgular: Alagebrium % gevseme yanitlari, endoteli saglam damarlarda endoteli bozulmus damarlara gore anlamli derecede artmistir. Vazodilatasyon ile ilgili kanallarin (KATP, PG12, BKca) ve reseptorlerin ([beta]1, [beta]2) bloke edilmesi de alagebrium un gevieme yanitini geri cevirememistir Alagebriuma vazodilator cevap L-NAME uygulanmasi ile hafif derecede azalirken BAYK-8644 uygulanmasi ile anlamli derecede azalmistir Sonuc: Calismamizin sonuclari; alagebriuma bagli vazodilator etki mekanizmasinin L-tipi kalsiyum kanal blokaji ve kismen de nitrik ok