Micronuclei frequencies in lymphocytes and cervical cells of women with polycystic ovarian syndrome/Polikistik over sendromlu kadinlarda lenfositler ve servikal hucrelerde mikronukleus frekanslari

• Published in: Turkish journal of obstetrics and gynecology 2017-09, Vol.14 (3), p.151
• Main Authors: Karatayli, Rengin, Zamani, Ayse Gul, Gezginc, Kazim, Tuncez, Ebru, Soysal, Sema, Karanfil, Fikriye, Acar, Aynur, Yildirim, M. Selman
• Format: Article
• Language: English
• Subjects: Cervix uteri; Comparative analysis; Development and progression; Genetic aspects; Gynecological research; Health aspects; Lymphocytes; Micronucleus tests; Pathological histology; Polycystic ovary syndrome
• ISSN: 2149-9322
• DOI: 10.4274/tjod.10734

Objective: The aim of this study was to determine micronucleus (MN) frequencies in exfoliated cervical cells and peripheral blood lymphocytes of women with polycystic ovarian syndrome (PCOS). Materials and Methods: Fifteen patients with PCOS and 11 healthy control patients were included in the study. Cervical smears and peripheral blood were collected from all patients. Specimens were analyzed for MN frequencies and compared between the groups. In addition to MN, other nuclear anomalies connected with both genotoxicity and cytotoxicity were evaluated. Results: The MN frequencies in cervical smear and peripheral blood lymphocytes were compared in patients with PCOS and normal controls. There was no statistically significant difference between the groups regarding micronucleus frequency in peripheral blood lymphocytes (p=0.239). The mean MN scores in exfoliated cervical cells of patients with PCOS and normal controls were 1.19[+ or -]0.57 and 0.74[+ or -]0.34, respectively The difference regarding micronucleus frequencies in cervical cells was statistically significant between the groups (p=0.032). Conclusion: Although study group is small, our study results support that there is an increased micronucleus frequency in cervical exfoliated cells of PCOS patients; this is a determinant of genetic hazard in the disease. Keywords: Micronucleus tests, polycystic ovarian syndrome, cervical smears, lymphocytes, genotoxicity tests Amac: Bu calismanin amaci, polikistik over sendromlu (PKOS) kadinlarin eksfoliyatif servikal hucrelerinde ve periferik kan lenfositlerinde mikronukleus (MN) frekanslarini belirlemektir. Gerec ve Yontemler: PKOS'li 15 hasta ve 11 saglikli kontrol hastasi calismaya dahil edildi. Tum hastalardan servikal smearleri ve periferik kan toplandi. Numuneler, MN frekanslari acisindan analiz edildi ve gruplar arasinda karsilastirildi. Hem MN sikligi, hem de genotoksisite ve sitotoksite bagli diger nukleer anomaliler degerlendirildi. Bulgular: Servikal smear ve periferik kan lenfositlerinde MN frekanslari PKOS hastalari ve normal kontrollerde karsilastirildi. Periferik kan lenfositlerinde MN frekansi acisindan gruplar arasinda istatistiksel olarak anlamli fark yoktu (p=0,239). PKOS hastalarinin eksfoliyatif servikal hucrelerindeki ortalama MN skorlari ve normal kontrollerinki sirasiyla 1,19[+ or -]0,57 ve 0,74[+ or -]0,34 idi. Servikal hucrelerdeki MN frekanslari istatistiksel olarak gruplar arasinda anlamliydi (p=0,032). Sonuc: Calisma grubu kucuk olma