CYTOTOXICITY AND GENOTOXICITY OF RESIN BASED DENTAL MATERIALS IN HUMAN LYMPHOCYTES IN VITRO/CITOTOKSICNI I GENOTOKSICNI UCINAK KOMPOZITNIH MATERIJALA NA STANICAMA LJUDSKIH LIMFOCITA IN VITRO

• Published in: Acta clinica Croatica (Tisak) 2018-06, Vol.57 (2), p.278
• Main Authors: Rajic, Valentina Brzovic, Zeljezic, Davor, Ivanisevic, Ana Malcic, Verzak, Zeljko, Baraba, Anja, Miletic, Ivana
• Format: Article
• Language: English
• Subjects: Apoptosis; Dental materials; Lymphocytes; Toxicity
• ISSN: 0353-9466
• DOI: 10.20471/acc.2018.57.02.07

The aim of this in vitro study was to evaluate cytotoxicity and genotoxicity of six different dental nanocomposite materials, three conventional ones and three flowable composite resin materials, in human lymphocytes. The following materials were tested: Tetric EvoCeram, Tetric Evo-Flow, Filtek Ultimate, Filtek Ultimate Flow, G-aenial and G-aenial Flo. Cytotoxicity was evaluated for two mass concentrations (0.007 g/mL and 0.013 g/mL) of each material, non-cured and cured, after 4 hours and 24 hours. Genotoxicity was evaluated using micronucleus assay under the same conditions as applied during the investigation of cytotoxicity. Uncured forms of Tetric EvoCeram, Tetric EvoFlow and Filtek Ultimate Flow in higher mass concentration caused genotoxic effect. Uncured G-aenial Flo in higher mass concentration induced apoptosis and necrosis. Uncured Tetric EvoFlow and uncured Filtek Ultimate Flow in higher mass concentration induced early apoptosis after both test periods. None of the conventional composite resin materials tested showed cytotoxicity except for uncured G-aenial, which induced apoptosis in higher mass concentration in both test periods. In conclusion, under the conditions of this in vitro study, cured conventional composites did not show cytotoxic or genotoxic effect, which is important for clinical application of these materials, whereas uncured forms exhibited certain level of cytotoxicity and genotoxicity, mainly because of monomers in their composition.Key words: Dental Materials; Nanocomposites; Composite Resins; Cytotoxins; ApoptosisSvrha ovoga istrazivanja bila je ispitati in vitro citotoksicni i genotoksicni ucinak sest razlicitih nanokompozitnih materijala, tri tekuca i tri konvencionalna kompozitna materijala na stanicama ljudskih limfocita. Istrazivanje je provedeno na materijalima: Tetric EvoCeram, Tetric EvoFlow, Filtek Ultimate, Filtek Ultimate Flow, G-aenial i G-aenial Flo. Ispitivan je citotoksicni ucinak dviju masenih koncentracija (0,007 g/mL i 0,013 g/mL) svakog ispitivanog polimeriziranog i nepolimeriziranog materijala nakon 4 sata i 24 sata. Genotoksicni ucinak je ispitivan primjenom mikronukleus testa u istim uvjetima kao sto je ucinjeno u ispitivanju citotoksicnog ucinka. Nepolimerizirani oblici materijala: Tetric EvoCeram, Tetric EvoFlow i Filtek Ultimate Flow u vecoj masenoj koncentraciji pokazali su genotoksicni ucinak. Nepolimerizirani G-aenial Flo je u vecoj masenoj koncentraciji izazvao apoptozu i nekrozu. U oba ispitiva