The synergistic effect of 2,3,5,4'-Tetrahydroxystilbene-2-O-[beta]-D-glucoside combined with Adriamycin on MCF-7 breast cancer cells

Objective: Breast cancer has been reported to be a serious disease and a threat to women's health. 2,3,5,4'-Tetrahydroxystilbene-2-O-P-D-glucoside (THSG) is a bioactive natural compound originating from Polygonum multiflorum Thunb., which has been shown to possess anti-inflammatory and ant...

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Bibliographic Details
Published in:Drug design, development and therapy development and therapy, 2018-01, Vol.10, p.4083
Main Authors: Shen, Jianfen, Zhang, Youzhi, Shen, Hui, Pan, Hua, Xu, Longsheng, Yuan, Linna, Ding, Zhiying
Format: Article
Language:English
Subjects:
Analysis
Anthracyclines
Anti-inflammatory agents
Breast cancer
Cancer cells
Cancer treatment
Chemotherapy
EDTA
Endothelium
Medical tests
Tumors
Women's health
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Summary:Objective: Breast cancer has been reported to be a serious disease and a threat to women's health. 2,3,5,4'-Tetrahydroxystilbene-2-O-P-D-glucoside (THSG) is a bioactive natural compound originating from Polygonum multiflorum Thunb., which has been shown to possess anti-inflammatory and antitumor properties. Adriamycin (ADM) is a chemotherapy agent used in tumor therapy that is limited by its side effects. However, little is known about the syner gistic effect of THSG combined with ADM on breast cancer. This study seeks to investigate the effects of the combination of THSG plus ADM on MCF-7 breast cancer cells and to test the mechanisms involved. Materials and methods: MTT assay was detected to determine cell viability. Furthermore, cell apoptosis was tested by flow cytometry and TUNEL assay. In addition, protein expression was measured by Western blot analysis. Results: The individual treatment of THSG and ADM induced cell injury. Moreover, cotreatment further increased it, which the effect may be associated with the elevation of the apoptotic-related protein expression such as Bax/Bcl-2 and cleaved caspase-3/caspase-3. Lastly, our results also show the reduction of vascular endothelial growth factor/phosphatidylinositol 3-kinase/Akt protein expression in the individual or synergistic treatment. Conclusion: Taken together, cotreatment of THSG and ADM may exert a synergistic reduction of cell injury via the inhibition of vascular endothelial growth factor/phosphatidylinositol 3-kinase/Akt pathway. Thus, THSG might possess potent anti-breast cancer effect with ADM. Keywords: THSG, ADM, synergistic effect, apoptosis, VEGF/PI3K/Akt, breast cancer
ISSN:1177-8881
1177-8881
DOI:10.2147/DDDT.S186028