Effect of salicylic acid and silver nitrate on rutin production by Hyptis marrubioides cultured in vitro/Efeito do acido salicilico e nitrato de prata na producao de rutina por Hyptis marrubioides cultivada in vitro

• Published in: Ciência rural 2019-02, Vol.49 (2)
• Main Authors: Pedroso, Rita Cassia Nascimento, de Avila Branquinho, Nubia Angelica, de Almeida Monteiro Hara, Alessandra Cristina Boffino, Silva, Fabiano Guimaraes, Filho, Luis Claudio Kellner, Silva, Marcio Luis Andrade, Cunha, Wilson Roberto, Pauletti, Patricia Mendonca, Januario, Ana Helena
• Format: Article
• Language: Spanish
• ISSN: 0103-8478
• DOI: 10.1590/0103-8478cr20180278

Hyptis marrubioides (Lamiaceae) is a medicinal plant that is native from Brazilian Cerrado. In vitro propagation techniques make use of elicitors to alter metabolic pathways, affecting how molecules are produced both qualitatively and quantitatively. This research aimed to evaluate how abiotic elicitors salicylic acid (SA) and silver nitrate (SN) at concentrations of 30 [micro]M or 60 [micro]M influence Hyptis marrubioides seedling growth by two different in vitro culture methods. The rutin content was quantified by HPLC-DAD. Compared to an untreated culture, the H. marrubioides methanolic extracts cultured in MS medium for 10 days followed by culture in MS medium containing SN (30 [micro]M) for 20 days had 1.28 times higher rutin content. In a second experiment, seedlings were cultured in MS medium for 20 days, and then the desired elicitor was added to the culture and allowed to remain in contact with the medium for three and six days. SA (30 [micro]M) gave the best results: rutin production was 16.56-fold higher than the control after six days. SN (30 [micro]M) increased the rutin content by 1.17-fold. At the two concentrations evaluated during the elicitation experiments, neither SA nor SN altered the growth parameters shoot length, leaf number, and fresh and dry weight of H. marrubioides seedlings grown in vitro as compared to the control. Based on these results, the abiotic elicitors SA and SN successfully provide Hyptis marrubioides with increased rutin content in vitro. Key words: salicylic acid, HPLC-DAD, tissue culture, silver nitrate, elicitation, plant growth regulators. Hyptis marrubioides (Lamiaceae) e uma planta medicinal nativa do Cerrado brasileiro. Tecnicas de propagacao in vitro fazem uso de elicitores para alterar as vias metabolicas, afetando a producao de moleculas qualitativa e quantitativamente. Este trabalho teve como objetivo avaliar como os elicitores abioticos acido salicilico (SA) e nitrato de prata (SN) nas concentracoes de 30 [micro]M ou 60 [micro]M influenciam no crescimento de plantulas de Hyptis marrubioides por dois diferentes metodos de cultivo in vitro. O teor de rutina foi quantificado por CLAEDAD. Em comparacao com uma cultura nao tratada, os extratos metanolicos de H. marrubioides cultivados em meio MS por 10 dias, seguidos de cultura em meio MS contendo SN (30 [micro]M) por 20 dias, apresentaram 1,28 vezes maior teor de rutina. Em um segundo experimento, as plantulas foram cultivadas em meio MS por 20 dias, e entao