The combination of catechin, baicalin and [beta]-caryophyllene potentially suppresses the production of inflammatory cytokines in mouse macrophages in vitro

It has been demonstrated that the combination of three botanical factors of (+)-catechin, baicalin and [beta]-caryophyllene, which exhibit anti-inflammatory effects, with comparatively lower concentrations of each factor, demonstrating a potent synergistic-suppressive effect on the growth of mouse m...

Full description

Saved in:
Bibliographic Details
Published in:Experimental and therapeutic medicine 2019-05, Vol.17 (5), p.4312
Main Authors: Yamaguchi, Masayoshi, Levy, Robert M
Format: Article
Language:English
Subjects:
Anti-inflammatory agents
Catechin
Cytokines
EDTA
Flavonoids
Laboratory rats
Macrophages
Medical research
Mitogens
Tumor necrosis factor
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:It has been demonstrated that the combination of three botanical factors of (+)-catechin, baicalin and [beta]-caryophyllene, which exhibit anti-inflammatory effects, with comparatively lower concentrations of each factor, demonstrating a potent synergistic-suppressive effect on the growth of mouse macrophage RAW264.7 cells in vitro, and suggesting it may function as a pharmacologic tool for managing inflammatory diseases. The present study was undertaken to determine the suppressive effects of (+)-catechin, baicalin or [beta]-caryophyllene on the production of inflammatory cytokines, including TNF-[alpha], IL-6 and IL-1[beta], which was enhanced by lipopolysaccharide (LPS) in RAW264.7 cells in vitro. The cells were cultured for 3 days without botanical factors, followed by incubation for 5 h in the presence of either vehicle, (+)-catechin [1 [micro]g/ml (3.45 [micro]M)], baicalin [1 [micro]g/ml (2.24 [micro]M)], or [beta]-caryophyllene [1 [micro]g/ml (5 [micro]M)] with or without LPS (100 ng/ml); this did not have significant effects on the number of RAW264.7 cells. The production of TNF-[alpha], IL-6 and IL-1[beta] was not altered by the addition of (+)-catechin, baicalin, [beta]-caryophyllene, or the three combined factors in RAW264.7 cells without LPS. LPS treatment caused a marked production of TNF-[alpha], IL-6, and IL-1[beta]. This enhancement was suppressed by the addition of (+)-catechin, baicalin or [beta]-caryophyllene. Of note, the production of these cytokines was additively suppressed by the combination of the three factors in macrophages. Thus, the combination of (+)-catechin, baicalin and [beta]-caryophyllene was found to reveal a potent suppressive effect on cytokine production in macrophages in vitro. This composition may be a useful tool as a potent anti-inflammatory agent. Key words: (+)-catechin, baicalin, [beta]-caryophyllene, TNF-[alpha], IL-6, IL-1[beta], macrophage, RAW264.7 cell
ISSN:1792-0981
DOI:10.3892/etm.2019.7452