Cortisol, progesterone, 17[alpha]-hydroxyprogesterone, and TSH responses in dogs injected with low-dose lipopolysaccharide

Background Stress and diseases such as endotoxemia induce cortisol synthesis through a complex biosynthetic pathway involving intermediates (progesterone, and 17[alpha]-hydroxyprogesterone (17[alpha]-OHP)) and suppression of the hypothalamus-pituitary-thyroid axis. Objective To measure plasma concen...

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Bibliographic Details
Published in:PeerJ (San Francisco, CA) CA), 2019-08, Vol.7, p.e7468
Main Authors: Corder-Ramos, Nicole L.B, Flatland, Bente, Fry, Michael M, Sun, Xiaocun, Fecteau, Kellie, Giori, Luca
Format: Article
Language:English
Subjects:
Dogs
Dosage and administration
EDTA
Glucocorticoids
Glycoproteins
Hormones
Hydroxyprogesterone
Mitogens
Pituitary hormones
Progesterone
Rankings
Sex hormones
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Summary:Background Stress and diseases such as endotoxemia induce cortisol synthesis through a complex biosynthetic pathway involving intermediates (progesterone, and 17[alpha]-hydroxyprogesterone (17[alpha]-OHP)) and suppression of the hypothalamus-pituitary-thyroid axis. Objective To measure plasma concentrations of cortisol, progesterone, 17[alpha]-OHP, and thyroid stimulating hormone (TSH) in dogs experimentally injected with intravenous low-dose lipopolysaccharide (LPS). Our hypothesis was that LPS treatment would elicit a significant increase in cortisol and its precursors, and a significant decrease in TSH concentration. Methods Hormone measurements were performed on blood samples left over from a previous investigation (2011) on the effect of low-dose LPS on hematological measurands. Five sexually intact female dogs, none in estrous at the time of the study, were administered saline treatment two weeks prior to LPS treatment. LPS was administered intravenously at a dose of 0.1 [micro]g/kg. Blood was collected before (baseline, time -24 hours) and 3-, 6- and 24-hours post-injection. Mixed model analysis for repeated measures was used, with both treatment and time as the repeated factors. Ranked transformation were applied when diagnostic analysis exhibited violation of normality and equal variance assumptions. Post hoc multiple comparisons were performed with Tukey's adjustment. Statistical significance was defined as p
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.7468