Effect of insulin-glucose metabolism compared with don adipose omentin gene expression in different models of diabetic C57BL/6 mice

• Published in: Diabetology and metabolic syndrome 2019-08, Vol.11 (1)
• Main Authors: Goodarzi, Golnaz, Shirgir, Amirreza, Alavi, Sadegh, Khoshi, Amirhosein
• Format: Article
• Language: English
• Subjects: Biochemistry; Blood glucose; Comparative analysis; Diabetes mellitus; Diabetes therapy; Diabetics; Gene expression; Genes; Genetic aspects; Glucose; Glucose metabolism; Health aspects; House mouse; Insulin; Messenger RNA; Obesity; Risk factors; RNA; Streptozocin; Type 1 diabetes; Type 2 diabetes
• ISSN: 1758-5996; 1758-5996
• DOI: 10.1186/s13098-019-0460-8

Background Omentin, releasing by adipose-tissue may be related to glucose metabolism. The omentin circulating levels and the related mRNA expression in visceral adipose-tissue are different in types of diabetes and the exact function of this molecule is still unknown. The aim of this study was to examine omentin gene expression in adipose-tissues of type-1 and type-2 diabetic mice for the investigation of the effects of fat-mass and insulin-glucose metabolism. Methods In this study, 36 C57BL/6 mice were divided into four experimental groups, including control, type-1 diabetes (inducted by streptozotocin), type-2 diabetes with obesity (high-fat diet + low-dose-streptozotocin [HFD + STZ]), and type-2 with normal weight (normal-pellet diet + low-dose-streptozotocin [NPD + STZ]). The present study involved the measurements of oral-glucose-tolerance-test and the levels of biochemical parameters, including blood glucose, omentin, insulin, lipid-profile, as well as aminotransferases. In addition, the omentin mRNA expression was evaluated by real-time polymerase-chain-reaction. Results The results of omentin gene expression analysis showed a significant difference between mRNA expressions in the experimental groups. The plasma omentin levels were significantly higher in type-1 diabetes group and lower in type-2 diabetes with NPD + STZ; however, the plasma omentin levels were not changed in the HFD + STZ group. In addition, the findings of serum-biochemical analysis revealed significant differences, compared to the control-group. Conclusions The omentin expression may be affected by insulin and glucose levels in different types of diabetes more than fat-mass, and due to the local activity, the serum omentin may not comply with its gene expression. Keywords: Omentin, Diabetes mellitus, Obesity, Insulin, C57BL/6 mice