Nanofibrillar cellulose wound dressing supports the growth and characteristics of human mesenchymal stem/stromal cells without cell adhesion coatings

In the field of regenerative medicine, delivery of human adipose-derived mesenchymal stem/stromal cells (hASCs) has shown great promise to promote wound healing. However, a hostile environment of the injured tissue has shown considerably to limit the survival rate of the transplanted cells, and thus...

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Published in:Stem cell research & therapy 2019-09, Vol.10 (1), p.292-13, Article 292
Main Authors: Kiiskinen, Jasmi, Merivaara, Arto, Hakkarainen, Tiina, Kääriäinen, Minna, Miettinen, Susanna, Yliperttula, Marjo, Koivuniemi, Raili
Format: Article
Language:English
Subjects:
Actin
Adipose-derived mesenchymal stem/stromal cell
Cell adhesion
Cell adhesion & migration
Cell culture
Cell density
Cell differentiation
Cell scaffold
Cell survival
Cell transplantation
Cell viability
Cellulose
Coatings
Collagen
Collagen (type I)
Cytology
Cytoskeleton
Cytotoxicity
DNA polymerases
Electron microscopy
Experiments
Extracellular matrix
Fibronectin
Fibronectins
Flow cytometry
Growth factors
Hospitals
Hydrogels
Immunocytochemistry
Mesenchyme
Microscopy
Morphology
Muscle proteins
Nanofibrillar cellulose
Physical characteristics
Plastic surgery
Polysaccharides
Proteins
Regenerative medicine
RNA-directed DNA polymerase
Scanning electron microscopy
Stem cells
Stromal cells
Surgery
Transplantation
Tumor necrosis factor-TNF
Vimentin
Wound care
Wound healing
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cited_by cdi_FETCH-LOGICAL-c552t-5214e3d4cece837daceb9eb8b676bae1839544d3c2494d379b49e69e8727ef6a3
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description In the field of regenerative medicine, delivery of human adipose-derived mesenchymal stem/stromal cells (hASCs) has shown great promise to promote wound healing. However, a hostile environment of the injured tissue has shown considerably to limit the survival rate of the transplanted cells, and thus, to improve the cell survival and retention towards successful cell transplantation, an optimal cell scaffold is required. The objective of this study was to evaluate the potential use of wood-derived nanofibrillar cellulose (NFC) wound dressing as a cell scaffold material for hASCs in order to develop a cell transplantation method free from animal-derived components for wound treatment. Patient-derived hASCs were cultured on NFC wound dressing without cell adhesion coatings. Cell characteristics, including cell viability, morphology, cytoskeletal structure, proliferation potency, and mesenchymal cell and differentiation marker expression, were analyzed using cell viability assays, electron microscopy, immunocytochemistry, and quantitative or reverse transcriptase PCR. Student's t test and one-way ANOVA followed by a Tukey honestly significant difference post hoc test were used to determine statistical significance. hASCs were able to adhere to NFC dressing and maintained high cell survival without cell adhesion coatings with a cell density-dependent manner for the studied period of 2 weeks. In addition, NFC dressing did not induce any remarkable cytotoxicity towards hASCs or alter the morphology, proliferation potency, filamentous actin structure, the expression of mesenchymal vimentin and extracellular matrix (ECM) proteins collagen I and fibronectin, or the undifferentiated state of hASCs. As a result, NFC wound dressing offers a functional cell culture platform for hASCs to be used further for in vivo wound healing studies in the future.
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However, a hostile environment of the injured tissue has shown considerably to limit the survival rate of the transplanted cells, and thus, to improve the cell survival and retention towards successful cell transplantation, an optimal cell scaffold is required. The objective of this study was to evaluate the potential use of wood-derived nanofibrillar cellulose (NFC) wound dressing as a cell scaffold material for hASCs in order to develop a cell transplantation method free from animal-derived components for wound treatment. Patient-derived hASCs were cultured on NFC wound dressing without cell adhesion coatings. Cell characteristics, including cell viability, morphology, cytoskeletal structure, proliferation potency, and mesenchymal cell and differentiation marker expression, were analyzed using cell viability assays, electron microscopy, immunocytochemistry, and quantitative or reverse transcriptase PCR. Student's t test and one-way ANOVA followed by a Tukey honestly significant difference post hoc test were used to determine statistical significance. hASCs were able to adhere to NFC dressing and maintained high cell survival without cell adhesion coatings with a cell density-dependent manner for the studied period of 2 weeks. In addition, NFC dressing did not induce any remarkable cytotoxicity towards hASCs or alter the morphology, proliferation potency, filamentous actin structure, the expression of mesenchymal vimentin and extracellular matrix (ECM) proteins collagen I and fibronectin, or the undifferentiated state of hASCs. 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However, a hostile environment of the injured tissue has shown considerably to limit the survival rate of the transplanted cells, and thus, to improve the cell survival and retention towards successful cell transplantation, an optimal cell scaffold is required. The objective of this study was to evaluate the potential use of wood-derived nanofibrillar cellulose (NFC) wound dressing as a cell scaffold material for hASCs in order to develop a cell transplantation method free from animal-derived components for wound treatment. Patient-derived hASCs were cultured on NFC wound dressing without cell adhesion coatings. Cell characteristics, including cell viability, morphology, cytoskeletal structure, proliferation potency, and mesenchymal cell and differentiation marker expression, were analyzed using cell viability assays, electron microscopy, immunocytochemistry, and quantitative or reverse transcriptase PCR. Student's t test and one-way ANOVA followed by a Tukey honestly significant difference post hoc test were used to determine statistical significance. hASCs were able to adhere to NFC dressing and maintained high cell survival without cell adhesion coatings with a cell density-dependent manner for the studied period of 2 weeks. In addition, NFC dressing did not induce any remarkable cytotoxicity towards hASCs or alter the morphology, proliferation potency, filamentous actin structure, the expression of mesenchymal vimentin and extracellular matrix (ECM) proteins collagen I and fibronectin, or the undifferentiated state of hASCs. 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subjects Actin
Adipose-derived mesenchymal stem/stromal cell
Cell adhesion
Cell adhesion & migration
Cell culture
Cell density
Cell differentiation
Cell scaffold
Cell survival
Cell transplantation
Cell viability
Cellulose
Coatings
Collagen
Collagen (type I)
Cytology
Cytoskeleton
Cytotoxicity
DNA polymerases
Electron microscopy
Experiments
Extracellular matrix
Fibronectin
Fibronectins
Flow cytometry
Growth factors
Hospitals
Hydrogels
Immunocytochemistry
Mesenchyme
Microscopy
Morphology
Muscle proteins
Nanofibrillar cellulose
Physical characteristics
Plastic surgery
Polysaccharides
Proteins
Regenerative medicine
RNA-directed DNA polymerase
Scanning electron microscopy
Stem cells
Stromal cells
Surgery
Transplantation
Tumor necrosis factor-TNF
Vimentin
Wound care
Wound healing
title Nanofibrillar cellulose wound dressing supports the growth and characteristics of human mesenchymal stem/stromal cells without cell adhesion coatings
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