Integrative analysis of the IncRNA-associated ceRNA network reveals lncRNAs as potential prognostic biomarkers in human muscle-invasive bladder cancer

Background: Long noncoding RNAs (lncRNAs) play important roles in competing endogenous RNA (ceRNA) networks involved in the development and progression of various cancers, including muscle-invasive bladder cancer (MIBC). Purpose: This study aims to construct the lncRNA-associated ceRNA network and i...

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Bibliographic Details
Published in:Cancer management and research 2019-07, p.6061
Main Authors: Lyu, Lei, Xiang, Wei, Zhu, Jin-Yan, Huang, Tao, Yuan, Jing-Dong, Zhang, Chuan-Hua
Format: Article
Language:English
Subjects:
Antisense RNA
Biological markers
Bladder cancer
Cancer genetics
Cancer research
Cell differentiation
Computational biology
Development and progression
Genes
Genetic aspects
Genomes
Genomics
Messenger RNA
MicroRNA
Necrosis
Polymerase chain reaction
RNA
Tumors
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Summary:Background: Long noncoding RNAs (lncRNAs) play important roles in competing endogenous RNA (ceRNA) networks involved in the development and progression of various cancers, including muscle-invasive bladder cancer (MIBC). Purpose: This study aims to construct the lncRNA-associated ceRNA network and identify lncRNA signatures correlated with the clinical features of MIBC tissue samples from The Cancer Genome Atlas (TGCA) database. Methods: The differential expression profiles of MIBC associated lncRNAs, miRNAs and mRNAs were obtained from TCGA. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to determine the principal functions of significantly dysregulated mRNAs. The dysregulated lncRNA-associated ceRNA network of MIBC was constructed based on the bioinformatics data, and the correlations between lncRNA expression and clinical features were analyzed using a weighted gene coexpression network analysis (WGCNA). Six cancer specific lncRNAs from the ceRNA network were randomly selected to detect their expression in 32 paired MIBC tissue samples and 5 bladder cancer cell lines using quantitative real-time polymerase chain reaction (qRT-PCR). Results: The ceRNA network was constructed with 30 lncRNAs, 13 miRNAs and 32 mRNAs. Seventeen lncRNAs in the ceRNA network correlated with certain clinical features, and only 1 lncRNA (MIR137HG) correlated with the overall survival (OS) of patients with MIBC (log-rank test P
ISSN:1179-1322
1179-1322
DOI:10.2147/CMAR.S207336