Principal Component Analysis-based Strategy to Identify the Presence of Tissue-Cultured and Wild-Type Dendrobium officinale and Dendrobium moniliforme in a Commercial Preparation

• Published in: Current topics in nutraceuticals research 2019-11, Vol.17 (4), p.355-362
• Main Authors: Chen, Nai-dong, Chen, Yue, Zhang, Li, Bao, Lin-Lin, Chen, Yan-Jun, Gu, Fang-Li, Wang, Fang, Jiang, Xue-Ping
• Format: Article
• Language: English
• Subjects: Analysis; Infrared spectroscopy; Scientific equipment industry; Spectroscopy
• ISSN: 1540-7535
• DOI: 10.37290/ctnr2641-452X.17:355-362

A principal component analysis-based two-dimensional correlation infrared spectroscopy technique combined with tri-step infrared macro-fingerprinting was explored to discriminate 1-4 years of tissue-cultured from wild-type Dendrobium officinale and Dendrobium moniliforme. The results showed that although speciation and origins of the Dendrobium samples could be differentiated by Fourier-transform infrared spectroscopy and second derivative infrared spectroscopy spectra, it is quite difficult to identify the age of Dendrobiums. In the further principal component analysis-based two-dimensional correlation infrared analysis, the range 1390-1230 [cm.sup.-1] and 1180-1020 [cm.sup.-1], in which the auto-peaks attributed a major variance ratio, were chosen to discriminate different cultivated years of Dendrobiums. Different ages of D. officinale and D. moniliforme showed their characteristic auto-peaks and cross-peaks with different peak positions and intensity in these two range. Our research indicated that principal component analysis-based strategy combined with tri-step infrared macro-fingerprint is suitable to rapidly discriminate the different ages of Dendrobiums with species and origins. Keywords: Dendrobium moniliforme, Dendrobium officinale, Principal component analysis